Graphene-based pipette tip solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry for determination of ten lipophilic shellfish toxins in shellfish

doi:10.3724/SP.J.1123.2018.11042

Abstract

Abstract:

A method for the simultaneous determination of ten lipophilic shellfish toxins was established based on graphene-based pipette tip solid-phase extraction (G-PT-SPE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The factors influencing the extraction efficiency, including the types of extractants, the amount of graphene, the types and volumes of washing and eluent solvents, were optimized in detail. Under the optimal conditions, the calibration curves showed linear relationships between the LC peak areas of the selected ion-pairs and the mass concentrations of the ten lipophilic shellfish toxins with correlation coefficients >0.99. The limits of detection (LODs) and the limits of quantification (LOQs) of the method were 0.1-1.1 μg/kg and 0.3-3.2 μg/kg, respectively. The recoveries of the ten lipophilic shellfish toxins spiked in blank oyster at three levels ranged from 72.0% to 101.2% with relative standard deviations <15%. The method is sensitive, simple, and effective, and is suitable for the determination of the lipophilic shellfish toxins in shellfish products.

Key words: graphene, lipophilic shellfish toxins, liquid chromatography-tandem mass spectrometry (LC-MS/MS), pipette tip solid-phase extraction, shellfish

CLC Number:

O658

YANG Xiao, HUANG Huawei, WU Yuan'an, WAN Yiwen, LI Xiaoling, HUANG Xiangrong. Graphene-based pipette tip solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry for determination of ten lipophilic shellfish toxins in shellfish[J]. Chinese Journal of Chromatography, 2019, 37(5): 505-511.

References

[1] Tan Z J, Wu H Y, Guo M M, et al. Journal of Fishery Sciences of China, 2013, 20(2):467 谭志军, 吴海燕, 郭萌萌, 等. 中国水产科学, 2013, 20(2):467
[2] European Commission. Regulation (EC) No 853/2004 of the European Parliament and of the Council of 29 April 2004 Laying Down Specific Hygiene Rules for Food of Animal Origin. Off J Eur Communities, 2004, L226:22
[3] Guo M M, Tan Z J, Wu H Y, et al. Chinese Journal of Chromatography, 2012, 30(3):256 郭萌萌, 谭志军, 吴海燕, 等. 色谱, 2012, 30(3):256
[4] Yao J H, Tan Z J, Zhou D Q. Chinese Journal of Analytical Chemistry, 2010, 38(12):1714 姚建华, 谭志军, 周德庆. 分析化学, 2010, 38(12):1714
[5] Huang C, Li X J, Peng R F, et al. Chinese Journal of Health Laboratory Technology, 2011, 21(5):1075 黄聪, 李晓晶, 彭荣飞, 等. 中国卫生检验杂志, 2011, 21(5):1075
[6] Liu R Y, Liang Y B, Liu L, et al. Ecology and Environmental Sciences, 2014, 23(8):1320 刘仁沿, 梁玉波, 刘磊, 等. 生态环境学报, 2014, 23(8):1320
[7] Zhang X Y, Cai X X. Chinese Journal of Health Laboratory Technology, 2012, 22(8):1905 张秀尧, 蔡欣欣. 中国卫生检验杂志, 2012, 22(8):1905
[8] Liu Q, Zeng G F, Wang Z Y, et al. Modern Food Science and Technology, 2015, 31(12):338 刘青, 曾广丰, 王志元, 等. 现代食品科技, 2015, 31(12):338
[9] Han S, Wang P Y, Liu Y, et al. Chinese Journal of Chromatography, 2013, 31(10):939 韩深, 王珮玥, 刘萤, 等. 色谱, 2013, 31(10):939
[10] Han S, Liu X, Li J H, et al. Food Science, 2014, 35(4):116 韩深, 刘鑫, 李建辉, 等. 食品科学, 2014, 35(4):116
[11] Hennion M C. J Chromatogr A, 1999, 856(1/2):3
[12] Poole C F. TrAC-Trends Anal Chem, 2003, 22(6):362
[13] Novoselov K S, Geim A K, Morozov S V, et al. Science, 2004, 306(5696):666
[14] Yang X, Feng B, He X L, et al. Microchim Acta, 2013, 180(11/12):935
[15] Wang L, Zang X H, Wang C, et al. Chinese J Anal Chem, 2014, 42(1):136
[16] Liu Q, Shi J, Jiang G. TrAC-Trends Anal Chem, 2012, 37(1):1
[17] Han Q, Wang Z H, Zhang X Q, et al. Progress in Chemistry, 2014, 26(5):820 韩强, 王宗花, 张晓琼, 等. 化学进展, 2014, 26(5):820
[18] Kaykhaii M, Mirmoghaddam M, Yahyavi H. Current Chromatogr, 2017, 4(1):4
[19] Yuan Y, Sun N, Yan H, et al. Microchim Acta, 2016, 183(2):799
[20] Sun N, Han Y, Yan H, et al. Anal Chim Acta, 2014, 810:25
[21] Yan H Y, Sun N, Liu S J, et al. Food Chem, 2014, 158:239
[22] Shi Z, Li Q, Xu D, et al. J Sep Sci, 2016, 39(22):4391
[23] Shen Q, Gong L, Baibado J T, et al. Talanta, 2013, 116(22):770
[24] Zhang H, Wu X, Yuan Y, et al. Food Chem, 2018, 265:290
[25] Jin R, Li L, Guo L, et al. Food Chem, 2017, 224:329
[26] Kaykhaii M, Yahyavi H, Hashemi M, et al. Anal Bioanal Chem, 2016, 408(18):4907