Chinese Journal of Chromatography ›› 2019, Vol. 37 ›› Issue (5): 525-532.DOI: 10.3724/SP.J.1123.2018.11016

• Articles • Previous Articles     Next Articles

Simultaneous determination of five types of 40 antibiotics in surface water using high performance liquid chromatography-tandem mass spectrometry

FENG Mengjuan1,2, YANG Zhengbiao3, ZHANG Qin2, SONG Ninghui2, LIU Yanhua1, GUO Ruixin1, CHEN Jianqiu1, ZHANG Shenghu2   

  1. 1. School of Engineering, China Pharmaceutical University, Nanjing 211198, China;
    2. Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China;
    3. Nanjing Environmental Monitoring Center Station, Nanjing 210013, China
  • Received:2018-11-11 Online:2019-05-08 Published:2015-03-23
  • Supported by:

    National Natural Science Foundation of China (Nos. 21876207, 21507165, 21407055).

Abstract:

A method was developed for the determination of five categories of 40 antibiotics in surface water by using solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS). The water samples were enriched and cleaned-up by filtration and with SPE cartridges. All antibiotics were separated by gradient elution with the mobile phase of 0.2% (v/v) formic acid aqueous solution and acetonitrile, and then analyzed with an electrospray ionization source in the positive ion and multiple reaction monitoring (MRM) modes. The results showed that the 40 antibiotics achieved great linearity in the range of 1-200 μg/L, and the average recoveries ranged from 41.3% to 112.6%. Using the developed method, thirteen antibiotics were identified in surface water from Nanjing section of the Yangtze River. The total antibiotic contents in the real samples ranged from 13.4 ng/L to 780.5 ng/L. The established method is efficient, sensitive and reliable, and is suitable for the determination of different antibiotics in real water samples.

Key words: antibiotics, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), solid phase extraction (SPE), surface water

CLC Number: