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Chinese Journal of Chromatography
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Chinese Journal of Chromatography  2019, Vol. 37 Issue (6): 589-596    DOI: 10.3724/SP.J.1123.2018.12011
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Authenticity identification of manuka honey using liquid chromatography-high resolution mass spectrometry based metabolomic technique
YAN Lijuan1, XU Dunming1, XUE Xiaofeng2, LIN Liyi1, LAI Kang3, WANG Jianwen3, ZHANG Zhigang1
1. Inspection and Quarantine Technology Center, Xiamen Customs, Xiamen 361026, China;
2. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China;
3. Sichuan Province Apiculture Management Station, Chengdu 610042, China
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Abstract 

A liquid chromatography-high resolution mass spectrometry (LC-HRMS) based metabolomic approach for the discrimination of manuka honey from the domestic honey was developed. The results of multivariate data analysis showed significant discrimination of manuka honey from domestic honey. Furthermore, a partial least squares discrimination model was established for honey discrimination, enabling the correct identification of unknown testing samples. A total of 34 highly expressed metabolic markers were obtained from the developed discrimination model, including 3-phenyllactic acid and 4-hydroxybenzoic acid which had both been previously identified as manuka honey markers. The area under the curve of the constructed marker combination reached 0.99. The developed metabolomic approach provides a new method for the quality control of manuka honey.

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YAN Lijuan
XU Dunming
XUE Xiaofeng
LIN Liyi
LAI Kang
WANG Jianwen
ZHANG Zhigang
Key wordsliquid chromatography (LC)   high resolution mass spectrometry (HRMS)   metabolomics   manuka honey     
Received: 2018-12-06;
Fund:

National Key Research and Development Program of China (No. 2017YFC1601600); Natural Science Foundation of Fujian Province (No. 2016J01071); Science and Technology People-Benefit Project of Xiamen (No. 3502Z20174043); Social Development Guided Key Project of Fujian Province (No. 2017Y0077); Former General Administration of Quality Supervision, Inspection and Quarantine Project (No. 2016IK184).

Cite this article:   
YAN Lijuan,XU Dunming,XUE Xiaofeng et al. Authenticity identification of manuka honey using liquid chromatography-high resolution mass spectrometry based metabolomic technique[J]. Chinese Journal of Chromatography, 2019, 37(6): 589-596.
 
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