Determination of two alkaloids in Mongolian medicine Mauleri-Dabusi-4 soup by hollow fiber liquid phase micro-extraction coupled with ultra-high performance liquid chromatography
BAO Guirong1,2, MENG He3, LI Youxin2, BAO James Jianmin2
1. Department of Biochemistry Engineering, Hohhot Vocational College, Hohhot 010051, China;
2. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China;
3. College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, China
In this study, a method combining hollow fiber liquid phase micro-extraction (HF-LPME) and ultra-high performance liquid chromatography (UPLC) was developed for the determination of two alkaloids (piperine and piperlonguminine) in Mongolian medicine Mauleri-Dabusi-4 Soup. The parameters affecting the micro-extraction efficiency were evaluated and optimized. The optimum conditions were as follows:polyvinylidence fluoride HF (54% pore size); 10 g/L NaCl; 30 μ L octanol as extraction solvent; 173 r/min stirring rate of extraction; and 128 min extraction time. The extracted drug was detected by UPLC. The calibration curves obtained good linear relationship in the concentration ranges of 100-8500 and 8.3-5000 μg/L for piperine and piperlonguminine, respectively. The enrichment factors of the method for piperine and piperlonguminine were achieved to be 59 and 65. The limits of detection were 2.2 μg/L for piperine and 2.5 μg/L for piperlonguminine. The method was successfully applied for the determination of alkaloids in Mongolian medicine Mauleri-Dabusi-4.
Inner Mongolia Natural Science Foundation (No. 2018MS02007).
Cite this article:
BAO Guirong,MENG He,LI Youxin et al. Determination of two alkaloids in Mongolian medicine Mauleri-Dabusi-4 soup by hollow fiber liquid phase micro-extraction coupled with ultra-high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2019, 37(6): 644-648.