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Chinese Journal of Chromatography
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Chinese Journal of Chromatography  2019, Vol. 37 Issue (6): 661-665    DOI: 10.3724/SP.J.1123.2018.12040
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Simultaneous determination of vitamins B1, B2, B6, niacinamide and calcium pantothenate in compound vitamin B tablets by high performance quantitative capillary electrophoresis
HU Wenwen1, TAO Jianwei1, WANG Qingwei1, LI Jing2, YAN Chao2,3, XU Xu1
1. School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China;
2. Unimicro Technologies, Inc., Shanghai 201203, China;
3. Pharmacy School, Shanghai Jiaotong University, Shanghai 200240, China
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Abstract 

A high performance quantitative capillary electrophoresis method was developed for the simultaneous determination of vitamins B1, B2, B6, nicotinamide, and calcium pantothenate in vitamin B tablets using a quantitative capillary electrophoresis instrument. The samples were extracted ultrasonically with acetonitrile-water (20:80, v/v). The automatic high precision quantitative capillary electrophoresis instrument was used to realize quantitative injection through a 10 nL injection valve. The background electrolyte was selected as 40 mmol/L sodium borate buffer (pH 9.0), which was continuously supplied by a microfluidic injection pump. The working voltage was -10 kV. The detection wavelength of vitamins B1, B2, B6, and nicotinamide was selected as 280 nm, which was then changed to 210 nm to detect calcium pantothenate. The result showed good linearities between the peak area and the concentration of vitamins B1, B2, B6, nicotinamide, and calcium pantothenate in the correlation coefficients (r) range of 0.9968-0.9998. The limits of detection (LODs) were in the range of 2.5-36.0 mg/L. The average recoveries were 94.1%-98.9% with relative standard deviations (RSDs) as 1.3%-1.9%. The method is precise, reliable, and suitable for the simultaneous determination of vitamins B1, B2, B6, nicotinamide, and calcium pantothenate in a real compound vitamin B tablet.

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HU Wenwen
TAO Jianwei
WANG Qingwei
LI Jing
YAN Chao
XU Xu
Key wordscapillary electrophoresis (CE)   compound vitamin B tablets   content determination     
Received: 2018-12-28;
Cite this article:   
HU Wenwen,TAO Jianwei,WANG Qingwei et al. Simultaneous determination of vitamins B1, B2, B6, niacinamide and calcium pantothenate in compound vitamin B tablets by high performance quantitative capillary electrophoresis[J]. Chinese Journal of Chromatography, 2019, 37(6): 661-665.
 
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