Chinese Journal of Chromatography ›› 2019, Vol. 37 ›› Issue (8): 831-835.DOI: 10.3724/SP.J.1123.2019.04048

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Preparation of magnetic nanoparticles based on MIL-100(Fe) and its application in the enrichment of endogenous peptides

ZHAO Yameng, WANG Min, ZHANG Lingyi, LU Yuhao, ZHANG Weibing   

  1. School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
  • Received:2019-04-30 Online:2019-08-08 Published:2015-07-30
  • Supported by:
    National Natural Science Foundation of China (Nos. 21475044, 51472121).

Abstract: Core-shell magnetic nanoparticles, Fe3O4@MIL-100(Fe), were synthesized by layer-by-layer self-assembly. The final material, Fe3O4@MC, was obtained after high temperature calcination. Fe3O4@MC magnetic nanoparticles exhibited large pore size (17.78 nm) and high carbon content (6.79%). Thirty-three peptides of bovine serum albumin (BSA) digest were enriched by Fe3O4@MC magnetic nanomaterial. Fe3O4@MC magnetic nanomaterial also showed high selectivity for peptides when the mass ratio of BSA digest to BSA was 1:400. In addition, the nanomaterial material exhibited excellent enrichment performance for the endogenous peptides of human serum.

Key words: endogenous peptides, enrichment, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), metal-organic framework (MOFs)

CLC Number: