Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Analysis of Volatile Constituents from Leaves of Plants by Gas Chromatography/Mass Spectrometry with
Solid-Phase Microextraction

WANG Minglin1, QIAO Luqin2, ZHANG Li3, WU Liejun3, TIAN Hongxiao3

  

  1. 1.College of Food Science, Shandong Agricultural University, Taian 271018, China; 2.Research
    Institute of
    Forest Ecology, Environment and Protection, Chinese Academy of Forest, Beijing 100091, China;
    3.Chemical Industry Research Institute of Shandong Province, Jinan 250014, China

  • Received:2005-08-19 Revised:2006-01-17 Online:2006-07-30 Published:1986-12-25

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Abstract:

A method for analyzing volatile constituents from plant leaves with gas
chromatography/mass spectrometry (GC/MS) coupled with solid-phase microextraction (SPME) was
developed. The volatile compounds from the plant leaves inside a sealed flask maintained at 45 ℃
in a water bath were efficiently extracted with Polyacrylate (85 μm) SPME fibers prior to perform
GC/MS analysis. The GC/MS analysis indicated that the volatile compounds from the plant leaves
which is easy to be damaged by Tetraychus vienneis include relative large amounts of cis-3-hexenyl
ester acetic acid, cis-3-hexenyl ester butanoic acid and alpha-famesene. These compounds were
preliminarily confirmed to be accountable for attracting Tetraychus vienneis. This finding may lead
to identify biological species for preventing and treating Tetraychus vienneis.

Key words:
volatile constituents, gas chromatography （GC）, mass spectro-metry（MS）, plant leaves , solid-phase microextraction (SPME）