色谱

色谱

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毛细管阵列电泳技术用于L-谷氨酸消旋化反应的条件优化

刘开颖1*, 白吉玲2, 王利2   

  1. 1. 中国科学院大连化学物理研究所生物技术部, 辽宁 大连 116023; 2. 中国科学院大连化学物理研究所分子反应动力学国家重点实验室, 辽宁 大连 116023
  • 收稿日期:2009-08-24 修回日期:2009-10-24 出版日期:2010-03-28 发布日期:1981-03-25
  • 通讯作者: 刘开颖

Optimization of L-glutamic acid racemization reaction with capillary array electrophoresis

LIU Kaiying1*, BAI Jiling2, WANG Li2   

  1. 1. Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; 2. State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
  • Received:2009-08-24 Revised:2009-10-24 Online:2010-03-28 Published:1981-03-25
  • Contact: LIU Kaiying

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被引次数

4

摘要： L-谷氨酸消旋化反应对于制备光学纯D-谷氨酸具有重要的意义。本文利用532 nm激光诱导荧光共聚焦检测式毛细管阵列电泳仪研究了L-谷氨酸基于席夫碱中间物的消旋化反应,采用羧基四甲基罗丹明琥珀酰亚胺酯(TAMRA)荧光探针标记消旋化产物后,以含有2 mmol/L环糊精(β-CD)的100 mmol/L Tris-硼酸缓冲液(pH 10)为分离电解质进行毛细管阵列电泳分析,在该条件下TAMRA标记的谷氨酸对映体可以得到基线拆分。详细考察了醛的种类及用量、羧酸溶剂的种类、反应体系的含水量对消旋化的影响,结果表明L-谷氨酸在含20%(体积分数)水的乙酸溶剂中,以水杨醛作为催化剂(水杨醛与L-谷氨酸的物质的量比为0.2)的优化条件下可以快速地被消旋化。

关键词: L-谷氨酸, 毛细管阵列电泳, 消旋化

Abstract: L-Glutamic acid (Glu) racemization research is of great importance for the preparation of optical pure D-Glu. In the present work, we studied L-Glu racemization reaction through Schiff base intermediate by using capillary array electrophoresis (CAE) with 532 nm laser-induced confocal fluorescence detection. The racemization products were labeled by carboxytetramethylrhodamine succinimidyl ester (TAMRA) and analyzed in a 100 mmol/L Tris-borate buffer (pH 10) system containing 2 mmol/L β-CD by CAE. Under this condition, the TAMRA-Glu enantiomer can be completely separated. The influences of the type and dosage of the aldehyde, organic acids, as well as the water content in the reaction system on the racemization were examined in detail, and L-Glu was racemized quickly in the presence of 0.2 molar ratio of L-Glu to salicylaldehyde (as the catalyzer) in acetic acid containing 20%(v/v) water.

Key words: L-glutamic acid (Glu), racemization , capillary array electrophoresis (CAE)