Chinese Journal of Chromatography ›› 2010, Vol. 28 ›› Issue (09): 908-911.DOI: 10.3724/SP.J.1123.2010.00908

• Technical Notes • Previous Articles    

Determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography with evaporative light scattering detection

卢凤来, 刘金磊, 陈月圆, 吴建璋, 李典鹏*   

  1. Guangxi Institute of Botany, Chinese Academy of Sciences, Guilin 541006, China
  • Received:2010-06-28 Revised:2010-08-11 Online:2010-09-28 Published:2010-09-28

Abstract: A method for the determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) was established. The results showed that esculeoside A had good linearity in the range of 0.61~3.05 mg with the correlation coefficient of 0.9995. The average recoveries were 97.9%~104.8% with the relative standard deviations (RSD) ≤4.14% (n=5). The method is simple, sensitive and suitable for the analysis of esculeoside A and quality control for the raw material and extract of Lycopersicon esculentum MILL.

Key words: esculeoside A, evaporative light scattering detection (ELSD), Lycopersicon esculentum MILL, high performance liquid chromatography (HPLC)