Determination of avermectin, diclazuril, toltrazuril and metabolite residues in pork by high performance liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2011.00217

Abstract

Abstract: A method for the determination of avermectin, ivermectin, doramectin, moxidectin, eprinomectin, diclazuril, toltrazuril and its two metabolite residues in pork was developed using QuEChERS method with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The sample was extracted with acetonitrile and purified through QuEChERS method using ODS as the sorbent. The target compounds were separated on a Venusil ASB C18 column (150 mm×2.1 mm, 3.0 μm) and detected by HPLC-MS/MS. The linear ranges were 0.005~0.2 mg/L and the correlation coefficients were all above 0.990. The average recoveries and the relative standard deviations ranged from 73.2% to 91.5% and from 12% to 17% at the spiked levels of 0.005, 0.01 and 0.02 mg/kg for the 9 analytes in pork matrix. This method is reliable, and suitable for the determination of the residues of avermectin and related compounds in pork.

Key words: avermectin, diclazuril, pork, toltrazuril and its metabolites, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)

GONG Xiaoming*, SUN Jun, DONG Jing, YU Jinling, WANG Hongtao. Determination of avermectin, diclazuril, toltrazuril and metabolite residues in pork by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2011, 29(03): 217-222.

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