基于3种非天然糖代谢标记的分泌蛋白质组分析性能对比
毛源1, 郑江南2, 封顺1,*(
), 田瑞军2,*()
), 田瑞军2,*()
Comparison of the performance of secretome analysis based on metabolic labeling by three unnatural sugars
MAO Yuan1, ZHENG Jiangnan2, FENG Shun1,*(), TIAN Ruijun2,*()
), TIAN Ruijun2,*()
图1. 基于质谱分析的分泌蛋白富集
Fig. 1. Secreted protein enrichment based on MS analysis
a. structures of three sugar analogues and metabolic labeling of glycoproteins containing sialic acid, GlcNAc, GalNAc, mannose, galactose, and fucose; b. the experimental workflow.
UDP: uridine diposphate; GALE: galactose-4-epimerase; PNGase F: peptide N-glycosidase F; GlcNAc: N-acetylglucosamine; GalNAc: N-acetylgalactosamine; HexNAz: six carbon carbohydrate analogues.
