基于混合模式的高效液相色谱固定相研究进展

李世杰, 李振, 汤紫霞, 吕美, 王利涛

Research progress on high performance liquid chromatography stationary phases based on mixed mode

LI Shijie, LI Zhen, TANG Zixia, LYU Mei, WANG Litao

表3 HILIC/IEC混合模式固定相的信息及分离物质

Table 3 HILIC/IEC mixed-mode stationary phase information and separated substances

Stationary phase Functional ligand Hydrophobic interactions Ion-exchange interactions Samples Ref. Sil-PolyCOOH polyethylene maleic anhydride， SiO2-NH2 -COOH carboxyl ionization nucleosides， nucleic acid bases， carbohydrate compounds， quaternary ammonium salts ［53］ （AMPS）n-ImprSiO2 CPTMO， V-Im， AMPS -CONH， sulfonic acid imidazole， sulfonic acid sulfonamide drugs， flavonoids， various components in extracts of traditional chinese medicine ligustrum lucidum ［54］ DCC/SiO2 DAC， sodium chlorite，SiO2-NH2 -COOH -COOH nucleosides， alkaloids and aniline compounds ［55］ C22-A3 long chain alkyl， polyamine groups -NH2， -CONH -NH2 nucleosides， nucleotides， aromatic acid compounds， multiple organic and inorganic anions ［56］ TPHs-SiO2 H3L -CONH， -NH2， -OH -NH2 seven nucleosides and nucleotides， five types of aniline compounds ［57］