基于混合模式的高效液相色谱固定相研究进展

李世杰, 李振, 汤紫霞, 吕美, 王利涛

Research progress on high performance liquid chromatography stationary phases based on mixed mode

LI Shijie, LI Zhen, TANG Zixia, LYU Mei, WANG Litao

表4 RPLC/HILIC/IEC混合模式固定相信息及分离物质

Table 4 RPLC/HILIC/IEC mixed-mode stationary phase information and separated substances

Stationary phases Functional ligand Hydrophobic interactions Hydrophilic interactions Ion-exchange interactions Samples Ref. SiO2-MPS-CQDs CQDs， MPS， SiO2 alkyl -OH， -COOH imidazole cationic substituent alkylbenzenes， PAHs， nucleoside bases， sulfonamides， anionic compounds ［20］ Sil-SVC， Sil-SVC-NPA SVC， NPA benzene ring -COOH， -NH2 -NH2 hydrophobic analytes， hydrophilic nucleotides， bases and anions ［23］ SiO2@P（St-AA） styrene and acrylic acid as polymerization monomers， vinyltrimethoxysilane benzene ring -COOH -COOH alkylbenzene and polycyclic aromatic hydrocarbons， nucleosides and bases， organic bases and acids ［24］ SiO2@TpBD-（OH）2 COPs， Tp， BD-（OH）2 benzene ring -COOH， -C=N- imidazole cationic substituent benzene homologues， polycyclic aromatic hydrocarbons， bases， acidic organic compounds ［26］ PIm-Br， coPIm-VBS SiO2 polymerized imidazole ionic liquid benzene ring， alkyl imidazole cationic substituent -COOH， -SO3H nucleosides， inorganic ions and organic ions ［42］ Sil-IM-Br， Sil-IM-SAG IM-Br， IM-SAG， Sil-MPS benzene ring， alkyl -OH imidazole cationic substituent， sulfonate group organic chemistry， inorganic ions， vitamin ［43］ Sil-DCH-Im2 SiO2 polymerized imidazole ionic liquid imidazole ring -NH2， -CONH- imidazole cationic substituent hydrophilic substances， hydrophobic substances， acids and inorganic anionic substances ［44］ Sil-PEI-APH PEI， APH benzene ring -CONH， -OH -NHR2， -N+R3 alkylbenzenes， PAHs， nucleosides， flavonoids， aromatic amine ［58］ Sil-G1-AS， Sil-G2-AS BDDE， AS， ethylenediamine octadecylamine chain -OH， quaternary ammonium ions quaternary ammonium ions PAHs， alkylbenzene， nucleoside， vitamin， phenolic， aromatic amine， flavonoids compounds ［59］ DPE-DIL， BND-DIL DPE， BND benzene ring imidazole ring， -CONH， -OH imidazole cationic substituent nucleosides and bases， PAHs， food additives ［60］