色谱

色谱

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碱催化法衍生化气相色谱/质谱法分析青海湖裸鲤鱼油中的脂肪酸

薄海波1,2,王霞3,翟宗德3,李永民3,陈立仁1

  

  1. 1.Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences, Lanzhou 730000, China; 2.Qinghai Entry-Exit Inspection and Quarantine Bureau, Xining 810000, China; 3.Graduated School, the Chinese Academy of Sciences, Beijing 100039, China
  • 收稿日期:2005-03-09 修回日期:2005-11-21 出版日期:2006-03-30 发布日期:1987-06-25
  • 通讯作者: 薄海波

Analysis of Fatty Acids in Gymnocypris przewalskii Oil by Gas Chromatography/Mass Spectrometry with
Base-Catalyzed Transesterification

BO Haibo1,2, WANG Xia3, ZHAI Zongde3, LI Yongmin3, CHEN Liren1

  

  1. 1.Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences, Lanzhou 730000, China; 2.Qinghai Entry-Exit Inspection and Quarantine Bureau, Xining 810000, China; 3.Graduated School, the Chinese Academy of Sciences, Beijing 100039, China
  • Received:2005-03-09 Revised:2005-11-21 Online:2006-03-30 Published:1987-06-25
  • Contact: BO Haibo

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摘要：

用碱催化法将青海湖裸鲤鱼油甲酯化,以气相色谱/质谱法分析鱼油中的脂肪酸。青海湖裸鲤可食用部分中鱼油含量为25.13%。从鱼油中共鉴定出47种脂肪酸，包括直链、单支链、多支链饱和脂肪酸,单不饱和、多不饱和脂肪酸，环丙烷基、呋喃基脂肪酸等。不饱和脂肪酸含量为73.6%,其中多不饱和脂肪酸含量为25.4%,以C18∶2(4.9%),C18∶3(3.1%),C20∶4(1.3%),C20∶5(二十碳五烯酸（EPA）, 9.4%)和C22∶6(二十二碳六烯酸（DHA）, 6.7%)为主。单不饱和脂肪酸含量为48.2%,主要由C16∶1(20.3%),C18∶1(25.9%)构成。饱和脂肪酸含量为25.7%,主要有C14∶0(3.4%)，C16∶0 (19.4%)和C18∶0(1.1%)。青海湖裸鲤鱼油中还存在不常见的环丙烷基和呋喃基脂肪酸及多种奇数碳链和支链脂肪酸。因此,青海湖裸鲤是功能性脂肪酸的重要膳食来源。

关键词: 气相色谱/质谱, 青海湖裸鲤, 鱼油 , 脂肪酸

Abstract:

The composition of fatty acids (FA) in Gymnocypris przewalskii oil was identified and quantified by gas chromatography (GC)/electron impact (EI) mass spectrometry (MS). A base-catalyzed transesterification method was used to convert fatty acids to methyl esters. The lipids were extracted using petroleum ether and the total lipids in dried meat and skin of Gymnocypris przewalskii were about 25%. Forty-seven fatty acids were identified in the current study. Main types of fatty acids found in the oils were normal saturated, mono-branched, multi-branched, cyclopropane, furanoid, normal monounsaturated and polyunsaturated fatty acids. Saturated fatty acids were approximately 25.7% of the total, and the main components were C14∶0(3.4%), C16∶0 (19.4%) and C18∶0(1.1%). Unsaturated fatty acids were totally 73.6%, and the major components of monounsaturated fatty acids were C16∶1 (9) (19.8%), C18∶1 (9) (18.6%) and C18∶1 (11) (7.3%); polyunsaturated fatty acids were mainly composed of C18∶2 (9,12) (4.8%), C18∶3 (9,12,15) (3.1%), C20∶4 (5,8,11,14) (1.2%), C20∶5 (5,8,11,14,17) (EPA, 9.4%) and C22∶6 (4,7,10,13,16,19) (DHA, 6.7%). Especially, furyl-, cyclopropane- and several odd and branched chain fatty acids were found in Gymnocypris przewalskii oil. It is thus an important dietary resource of functional fatty acids.

Key words: fatty acids, fish oil , gas chromatography/mass spectrometry (GC/MS), Gymnocypris przewalskii