色谱

色谱

• 研究论文 • 上一篇    下一篇

快速气相色谱法测定食用油中的未衍生化长链脂肪酸

孟哲1,2,文大为1,廖一平1,刘虎威1   

  1. 1.College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;2.Department of Biochemistry, Xingtai University, Xingtai 054001, China
  • 收稿日期:2006-08-21 修回日期:2006-11-20 出版日期:2007-03-30 发布日期:1985-12-25
  • 通讯作者: 刘虎威

Determination of Underivatized  Long-Chain Fatty Acids in Edible Oils by Fast Gas Chromatography

MENG Zhe1,2, WEN Dawei1, LIAO Yiping1, LIU Huwei1   

  1. 1.College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;2.Department of Biochemistry, Xingtai University, Xingtai 054001, China
  • Received:2006-08-21 Revised:2006-11-20 Online:2007-03-30 Published:1985-12-25
  • Contact: LIU Huwei

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摘要： 建立了利用短的微径色谱柱直接测定食用油中未衍生化长链脂肪酸的快速气相色谱方法。C12~C22脂肪酸在2 min之内实现基线分离，定量标准曲线的相关系数大于0.988，最低检测限（S/N=3）为2.80~9.60 mg/L,回收率为74.5%~86.5%。该方法快速、简便、准确，分析通量大，已成功地用于香油、色拉油及调和油等3种食用油中长链脂肪酸含量的测定。

关键词: 长链脂肪酸, 非衍生化, 快速气相色谱法, 食用油

Abstract: A fast gas chromatography (GC) method with short micro-bore capillary column was developed for the determination of underivatized long-chain fatty acids (LCFAs) in edible oils. Baseline separation of eight kinds of LCFAs (dodecanoic acid, tetradecanoic acid, palmitic acid, oleic acid, stearic acid, eicosanic acid, erudcic acid, behenic acid) was achieved within 2 min with high resolution performance. The linear correlation coefficients for underivatized LCFAs were higher than 0.988 and detection limits (at signal-to-noise of 3∶1) were 2.80-9.60 mg/L. The method is easy and low-cost and has the ability to achieve an accurate determination of the LCFAs in three edible oils.

Key words: edible oils , long-chain fatty acids (LCFAs), underivatization, fast gas chromatography (FGC)