色谱

色谱

• 研究论文 • 上一篇    下一篇

超高效液相色谱法同时测定化妆品中的15种激素

马强，王超，王星，白桦，陈伟，武婷，周新，于文莲

  

  1. Institute of Industrial Product Inspection, Chinese Academy of Inspection and Quarantine, Beijing 100025, China
  • 收稿日期:2007-01-18 修回日期:2007-04-29 出版日期:2007-07-30 发布日期:1985-06-25
  • 通讯作者: 马强

Simultaneous Determination of 15 Hormones in Cosmetics Using Ultra Performance Liquid Chromatography

MA Qiang, WANG Chao, WANG Xing, BAI Hua, CHEN Wei, WU Ting, ZHOU Xin, YU Wenlian   

  1. Institute of Industrial Product Inspection, Chinese Academy of Inspection and Quarantine, Beijing 100025, China
  • Received:2007-01-18 Revised:2007-04-29 Online:2007-07-30 Published:1985-06-25

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摘要：

建立了采用超高效液相色谱同时测定化妆品中糖皮质激素、雌激素、雄激素、孕激素等共15种激素(曲安西龙、氢化可的松、泼尼松、可的松、甲基泼尼松龙、倍他米松、地塞米松、醋酸泼尼松龙、醋酸氢化可的松、雌三醇、雌二醇、雌酮、己烯雌酚、睾酮、孕酮)的分析方法。不同形态的化妆品样品均以甲醇为提取溶剂进行超声提取,经Oasis HLB固相萃取柱富集净化,以Waters ACQUITY UPLC BEH C18色谱柱(1.7 μm,2.1 mm×50 mm)分离,乙腈和水为流动相梯度洗脱,6 min 内完成了15种激素的分离及检测。在1～25 ng进样范围内,15种激素的工作曲线的线性相关系数r均高于0.9995。在低、中、高（2,10,20 mg/kg）3个添加水平下15种激素的平均回收率为88.2%～102.4%,相对标准偏差为1.6%～7.4%。

关键词: 超高效液相色谱法, 固相萃取, 化妆品 , 激素

Abstract:

An analytical method based on ultra performance liquid chromatography has been developed for the simultaneous determination of 15 hormones (triamcinolone, hydrocortisone, prednisone, cortisone, 6α-methylprednisolone, betamethasone, dexamethasone, prednisolone acetate, hydrocortisone acetate, estriol, β-estradiol, estrone, diethylstilbestrol, testosterone and progesterone) in cosmetics. Various cosmetic samples were extracted with methanol under ultrasonication, and then cleaned up by a Oasis HLB solid phase extraction cartridge. The 15 hormones were separated and detected within 6 min on a Waters ACQUITY UPLC BEH C18 column (1.7 μm, 2.1 mm×50 mm) by gradient elution with acetonitrile and water system. In the range of 1-25 ng, the calibration curves of 15 hormones showed good linearity with the correlation coefficients larger than 0.9995. The mean recoveries at the three spiked levels （2, 10, 20 mg/kg） were 88.2%-102.4%with the relative standard deviations of 1.6%-7.4%.

Key words: cosmetics , hormones, solid phase extraction (SPE), ultra performance liquid chromatography (UPLC)