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色谱

• 样品预处理方法专栏 • 上一篇    下一篇

加速溶剂萃取技术在中药有效成分分析中的应用

陈军辉1,2,杨佰娟1,李文龙1,王小如1,3,黎先春1 ,杨黄浩1

  

  1. 1.Qingdao Key Laboratory on Analytical Technology Development and Standardization of Traditional Chinese Medicines, The First Institute of Oceanography of State Oceanic Administration, Qingdao 266061, China; 2.College ofChemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China; 3.Department of Chemistry and the Key Laboratory of Analytical Science of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
  • 收稿日期:2007-08-14 修回日期:1900-01-01 出版日期:2007-09-30 发布日期:1985-03-25
  • 通讯作者: 王小如

Application of Accelerated Solvent Extraction Technique for Analysis of Active Components in Traditional Chinese Medicinal Herbs

CHEN Junhui1,2, YANG Baijuan1, LI Wenlong1, WANG Xiaoru1,3, LEE Frank Sen-Chun1, YANG Huanghao1   

  1. 1.Qingdao Key Laboratory on Analytical Technology Development and Standardization of Traditional Chinese Medicines, The First Institute of Oceanography of State Oceanic Administration, Qingdao 266061, China; 2.College ofChemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China; 3.Department of Chemistry and the Key Laboratory of Analytical Science of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
  • Received:2007-08-14 Revised:1900-01-01 Online:2007-09-30 Published:1985-03-25

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摘要：

以两种药材为研究实例,对加速溶剂萃取法（ASE）在中药材有效成分提取研究中的应用进行了简要介绍。采用正交试验法考察了提取丹参中丹酚酸B的提取条件（萃取温度、静态萃取时间、萃取溶剂以及料液比），确定了较好的实验条件。比较了ASE、水蒸气蒸馏法、超声波提取法及索氏提取法对木香挥发油的提取效果，结果表明ASE对木香挥发油的提取效果最好。

关键词: 丹参, 加速溶剂萃取法, 木香

Abstract:

The application of accelerated solvent extraction (ASE) technique for the Analysis of active components in traditional Chinese medicinal herbs was introduced by using two kinds of herbs as examples. The conditions including extraction temperature, static extraction time, the ratio of material to solvent and solvent of ASE for extraction of salvianolic acid B in Salvia miltiorrhiza were optimized by orthogonal experiments, and the optimal conditions were obtained. Different extraction methods (ASE, steam distillation, ultrasonic wave and Soxhlet extraction) were used to extract volatile oil in Aucklandia lappa Decne. Results of the comparative experiments indicated that ASE was the most effective method in this case. All the results from these studies demonstrate that ASE is indeed a powerful tool in the preparation of herbal extracts for downstream chromatographic analysis.

Key words: Aucklandia lappa Decne , Salvia miltiorrhiza, accelerated solvent extraction