色谱

色谱

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多维高效液相色谱技术在蛋白质分离研究中的进展

高明霞, 关霞, 洪广峰, 张祥民*   

  1. Department of Chemistry, Fudan University, Shanghai 200433, China
  • 收稿日期:2009-07-02 修回日期:2009-07-16 出版日期:2009-09-30 发布日期:1982-03-25
  • 通讯作者: 张祥民

Advances in multidimensional high performance liquid chromatography for separation technology in proteomic study

GAO Mingxia, GUAN Xia, HONG Guangfeng, ZHANG Xiangmin*   

  1. Department of Chemistry, Fudan University, Shanghai 200433, China
  • Received:2009-07-02 Revised:2009-07-16 Online:2009-09-30 Published:1982-03-25
  • Contact: ZHANG Xiangmin

PDF

1011

被引次数

19

摘要： 蛋白质组学出现之后,多维高效液相色谱(multidimensional HPLC,MD-HPLC)系统以其快速、高效、自动化程度高以及容易与质谱等其他技术联用等优势而成为蛋白质组学相关分析技术中研究应用的热点。本文主要以本实验室在蛋白质组学研究中的技术进展为主线,介绍了多维高效液相色谱技术的发展,包括经典的“bottom-up”技术和“top-down”式的多维高效液相色谱技术路线,以及为了提高系统的分离通量而自行设计搭建的阵列式多维高效液相色谱平台,这些技术路线在蛋白质组学研究中有着极大的潜在应用价值。

关键词: bottom-up, top-down, 蛋白质分离 , 多维液相色谱, 完整蛋白质, 阵列式

Abstract: With the developments of proteomic study, multidimensional high performance liquid chromatography technology has attracted increasing interests due to its obvious advantages, such as rapid analysis, high automation and easy combination with mass spectrometry and so on. This review emphasizes the advances of multidimensional high performance liquid chromatography technology, including classical bottom-up methods, top-down techniques and array-based two-dimensional liquid chromatography system, which was designed and set up by our lab to improve the throughput. These techniques showed promising potential applications in proteomics study.

Key words: array-based two-dimensional liquid chromatography, bottom-up, intact protein, protein separation , top-down, multidimensional liquid chromatography