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色谱

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高效毛细管电泳法检测牛奶中的青霉素中间体以及3种青霉素类药物

田春秋1, 檀华蓉2*, 高丽萍3, 沈虎琴1, 祁克宗1   

  1. 1. 安徽农业大学茶与食品科技学院, 安徽 合肥 230036; 2. 安徽农业大学生物技术中心, 安徽 合肥 230036; 3. 安徽农业大学生命科学学院, 安徽 合肥 230036
  • 收稿日期:2011-06-20 修回日期:2011-07-07 出版日期:2011-11-28 发布日期:2011-12-25
  • 通讯作者: 檀华蓉,副研究员,主要从事兽药残留与动物源性食品安全的研究.
  • 基金资助:

    “十一五”国家科技支撑项目(2006BAK02A08_4)

Determination of penicillin intermediate and three penicillins in milk by high performance capillary electrophoresis

TIAN Chunqiu1, TAN Huarong2*, GAO Liping3, SHEN Huqin1, QI Kezong1   

  1. 1. College of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, China; 2. Biotechnology Center, Anhui Agricultural University, Hefei 230036, China; 3. School of Life Science, Anhui Agricultural University, Hefei 230036, China
  • Received:2011-06-20 Revised:2011-07-07 Online:2011-11-28 Published:2011-12-25

PDF

566

被引次数

54

摘要： 建立了高效毛细管电泳(HPCE)同时检测牛奶中青霉素类抗生素中间体6-氨基青霉烷酸(6-APA)以及3种青霉素类药物青霉素钾(PEN)、氨苄青霉素(AMP)和阿莫西林(AMO)的方法。利用正交实验设计,对HPCE中的缓冲液离子浓度和pH值、分离电压、分离温度等分离条件进行了优化。结果表明: 在采用40 mmol/L磷酸二氢钾-20 mmol/L硼砂缓冲体系(pH 7.8)、分离电压为28 kV、分离温度为30 ℃的电泳条件下,4.5 min内可以实现上述4种青霉素类药物的快速分离检测。各组分在1.56～100 mg/L范围内有良好的线性,相关系数(r2)为0.9979～0.9998,加标回收率为84.91%～96.72%,相对标准偏差(RSDs)为1.11%～9.11% (n=6)。该方法简便、快速,可以应用于市售牛奶中4种青霉素类药物的快速检测。

关键词: 6-氨基青霉烷酸, 高效毛细管电泳, 牛奶, 青霉素类药物, 青霉素中间体

Abstract: A high performance capillary electrophoresis (HPCE) method was developed for the simultaneous determination of penicillin intermediate and penicillins in milk, including 6-amino-penicillanic acid (6-APA), penicillin G (PEN), ampicillin (AMP) and amoxicillin (AMO). The main parameters including the ion concentration and pH value of running buffer, separation voltage and column temperature were optimized systematically by orthogonal test. The four penicillins (PENs) were baseline separated within 4.5 min with the running buffer of 40 mmol/L potassium dihydrogen phosphate-20 mmol/L borax solution (pH 7.8), separation voltage of 28 kV and column temperature of 30 ℃. The calibration curves showed good linearity in the range of 1.56~100 mg/L, and the correlation coefficients (r2) were between 0.9979 and 0.9998. The average recoveries at three spiked levels were in the range of 84.91%~96.72% with acceptable relative standard deviations (RSDs) of 1.11%~9.11%. The method is simple, fast, accurate and suitable for the determination of penicillins in real samples.

Key words: 6-aminopenicillanic acid, milk, penicillin intermediate, penicillins, high performance capillary electrophoresis(HPCE)