色谱

色谱 ›› 2012, Vol. 30 ›› Issue (12): 1271-1275.DOI: 10.3724/SP.J.1123.2012.07024

• 研究论文 • 上一篇    下一篇

高效液相色谱法考察不同炮制方法对知母中5种主要化学成分的影响

赵路路1, 刘菲菲1, 彭缨1,2*, 康廷国2   

  1. 1. 沈阳药科大学中药学院, 辽宁 沈阳 110016； 2. 辽宁中医药大学, 辽宁 沈阳 110032
  • 收稿日期:2012-07-17 修回日期:2012-08-23 出版日期:2012-12-28 发布日期:2012-12-19
  • 通讯作者: 彭缨,副教授,主要研究方向为中药药效物质基础及质量控制研究. E-mail: yingpeng1999@yahoo.com.cn.
  • 基金资助:

    辽宁省博士启动基金项目(20111139)和辽宁省高等学校杰出青年学者成长计划资助项目(LJQ2011105).

Effects of different processing methods on five main chemical constituents of Anemarrhena asphodeloides Bge. studied by high performance liquid chromatography

ZHAO Lulu1, LIU Feifei1, PENG Ying1,2*, KANG Tingguo2   

  1. 1. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China
  • Received:2012-07-17 Revised:2012-08-23 Online:2012-12-28 Published:2012-12-19

PDF

425

被引次数

22

摘要： 建立了高效液相色谱(HPLC)同时测定知母不同炮制品中新芒果苷、芒果苷、知母皂苷BIII、知母皂苷I和知母皂苷AIII含量的方法,探讨了不同炮制方法对知母化学成分的影响,为通过采用不同炮制方法来改变知母药性提供了依据。采用AlltimaTM C18色谱柱(250 mm×4.6 mm, 5 μm)分离,所用流动相为乙腈(A)和0.1%甲酸水溶液(B),梯度洗脱,流速为0.8 mL/min;用紫外检测器检测新芒果苷与芒果苷,检测温度为室温,检测波长为265 nm。用蒸发光散射检测器检测知母皂苷BIII、知母皂苷I和知母皂苷AIII,漂移管温度为50 ℃,气体压力为179.1 kPa(26 psi)。知母经炮制后,5种化合物含量均发生了不同程度的改变,表明炮制方法的不同对知母化学成分的含量影响较大。该方法对进一步研究炮制方法对知母的药效学影响提供了一定的参考依据。

关键词: 高效液相色谱, 芒果苷, 炮制, 新芒果苷, 知母, 知母皂苷AIII, 知母皂苷BIII, 知母皂苷I

Abstract: A high performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of neomangiferin, mangiferin, timosaponin BIII, anemarrhenasaponin I and timosaponin AIII in the products of Anemarrhena asphodeloides Bge. processed by different methods. By comparing and analyzing the variation of the contents of the five components, the effects of processing on chemical constituents of Anemarrhena asphodeloides Bge. were explored, which provided evidences for the relevance between processing and the property changes of Anemarrhena asphodeloides Bge. The chromatographic separation was performed on an AlltimaTM C18 column (250 mm×4.6 mm, 5 μm) with a gradient elution of acetonitrile (A) and 0.1% formic acid (B) (0~10 min, 5%A~20%A; 10~15 min, 20%A~25%A; 15~30 min, 25%A~80%A; 30~35 min, 80%A~100%A) at a flow rate of 0.8 mL/min. Neomangiferin and mangiferin were detected by an ultraviolet detector at 265 nm and room temperature. Timosaponin BIII, anemarrhenasaponin I and timosaponin AIII were detected by an evaporative light scattering detector with the drift temperature at 50 ℃ and gas pressure at 179.1 kPa (26 psi). To some extent, the contents of the major components varied in different processed products of Anemarrhena asphodeloides Bge. The results indicated that different processing methods caused significant differences in the contents of the major components of Anemarrhena asphodeloides Bge. It is of great use for further researching the relevance of the processing methods to pharmacodynamics of Anemarrhena asphodeloides Bge.

Key words: Anemarrhena asphodeloides Bge., anemarrhenasaponinI, mangiferin, neomangiferin, processing, timosaponin AIII, timosaponin BIII, high performance liquid chromatography (HPLC)

中图分类号: