色谱

色谱 ›› 2014, Vol. 32 ›› Issue (1): 100-104.DOI: 10.3724/SP.J.1123.2013.08008

• 技术与应用 • 上一篇    

斜投影-不展开薄层色谱法对废木料液化产物乙酰丙酸的过程分析

粟晖1, 葛军1, 方凤1, 姚志湘1, 宋光均2   

  1. 1. 广西科技大学生物与化学工程学院, 广西 柳州 545006;
    2. 广州标旗电子科技有限公司, 广东 广州 510640
  • 收稿日期:2013-08-13 修回日期:2013-11-14 出版日期:2014-01-08 发布日期:2013-12-26
  • 通讯作者: 姚志湘
  • 基金资助:

    广西科技计划项目(桂科能129825-20)；广西科技计划项目(桂科攻1355010-15)；广西研究生创新计划项目(2012105940817M04).

Process analysis for levulinic acid generated in waste wood liquefaction by non-development thin-layer chromatography based on oblique projection

SU Hui1, GE Jun1, FANG Feng1, YAO Zhixiang1, SONG Guangjun2   

  1. 1. College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China;
    2. Guangzhou Biaoqi Electronic Science and Technology Co., Ltd., Guangzhou 510640, China
  • Received:2013-08-13 Revised:2013-11-14 Online:2014-01-08 Published:2013-12-26

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摘要：

采用多波长薄层色谱扫描法，不经过传统的薄层色谱展开步骤实现混合体系的快速定量。废木料液化过程产物为混合体系，分别将不同液化反应时间点所取样品点样于硅胶板上。仅对液化过程终点的样本点进行一次展开，分离斜投影建模所需的反射光谱，切割出目标产物(乙酰丙酸)和背景光谱，构造斜投影算子；对其他液化过程中的样品点不展开，采集混合光谱，经斜投影算法分离出其中的目标产物纯光谱，从而实现定量。将该方法的定量结果与高效液相色谱法的定量结果对比，得到两种方法测定乙酰丙酸的相对误差小于3.27%，表明两种方法具有良好的一致性。

关键词: 不展开薄层色谱, 多波长薄层色谱扫描, 废木料, 斜投影, 液化过程, 乙酰丙酸

Abstract:

A rapid and quantitative method is presented for multi-component process analysis, based on multi-wavelength thin-layer chromatography (TLC) scanning but without the routine development. The samples from the waste wood liquefaction process are applied on silica plates, and just the last sample of spot need to be developed for getting separated spectra. These spectra are divided into two parts of production (levulinic acid) and background, respectively, to build an oblique projection operator. The other process samples do not need to be developed repeatedly, and are scanned to collect hybrid spectra immediately. The pure production spectrum can be separated from the process spectrum by the oblique projection algorithms to realize the production quantification. It was showed that the relative errors between the determination results by this method and those by high performance liquid chromatography (HPLC) were less than 3.27%, and so the consistency is perfect.

Key words: levulinic acid, liquefaction process, multi-wavelength thin layer chromatography (TLC) scanning, non-development thin layer chromatography(NDTLC), oblique projection, waste wood

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