色谱 ›› 2016, Vol. 34 ›› Issue (12): 1250-1254.DOI: 10.3724/SP.J.1123.2016.09006

• 研究论文 • 上一篇    下一篇

一种新型核壳型色谱固定相在高效液相色谱中的评价及应用

孙元社1,2, 瞿其曙3, 于淑新1,2, 李霜霜1, 唐涛1,2, 李彤1,2   

  1. 1. 大连依利特分析仪器有限公司, 辽宁 大连 116023;
    2. 辽宁大连依利特分析仪器工程技术研究中心, 辽宁 大连 116023;
    3. 安徽建筑大学材料与化学工程学院, 安徽 合肥 230601
  • 收稿日期:2016-09-01 出版日期:2016-12-08 发布日期:2013-04-24
  • 通讯作者: 李彤,Tel:(0411)84732388,E-mail:tonglii@eliteHPLC.com
  • 基金资助:

    国家重大科学仪器设备开发专项(2012YQ12004403);国家自然科学基金(51472121);大连市科技计划项目(2015A11GX028).

Evaluation and application of a new core-shell chromatographic stationary phase for high performance liquid chromatography

SUN Yuanshe1,2, QU Qishu3, YU Shuxin1,2, LI Shuangshuang1, TANG Tao1,2, LI Tong1,2   

  1. 1. Dalian Elite Analytical Instruments Co., Ltd., Dalian 116023, China;
    2. Liaoning Engineering Research Center of Dalian Elite Analytical Instruments, Dalian 116023, China;
    3. School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei 230601, China
  • Received:2016-09-01 Online:2016-12-08 Published:2013-04-24
  • Supported by:

    National Major Scientific Instrument and Equipment Development Project (No. 2012YQ12004403); National Natural Science Foundation of China (No. 51472121); Dalian Municipal Science and Technology Plan Project (No. 2015A11GX028).

摘要:

采用扫描电镜、透射电镜和氮气吸附法对制备的新型核壳型色谱固定相进行了表征,结果表明该固定相单分散性好、表面放射状孔道壳层结构均一、孔径分布窄。对该核壳材料的表面进行C18键合修饰,考察其基本色谱性能,色谱柱的理论塔板数超过150000块/m,色谱峰峰形对称,甲苯与乙苯的保留因子之比为1.45,亚甲基选择性优异。将该核壳材料应用于汽车尾气中醛酮类化合物的检测,在优化的色谱条件下,2,4-二硝基苯肼(DNPH)衍生的醛酮类化合物在15 min内获得了较好的分离效果。该核壳型C18色谱固定相具有分离速度快、选择性好、柱效高等特点,适于复杂样品的高效、快速分离分析。

关键词: 高效液相色谱, 核壳型色谱固定相, 评价与应用, 醛酮类化合物

Abstract:

A new core-shell chromatographic stationary phase was characterized by scanning electron microscope, transmission electron microscope and nitrogen adsorption method. The results showed that it had excellent monodispersity, homogeneous structure and narrow pore size distribution. It was modified by C18, and the chromatographic performance was also performed. The number of theoretical plates was over 150000 plates/m, and the chromatographic peaks were symmetrical. The retention factor ratio of toluene and ethylbenzene was 1.45, and the methylene selectivity was excellent. Finally, it was applied to detect aldehydes and ketones in automobile exhaust. The aldehydes and ketones which derivatized by 2,4-dinitrophenylhydrazine (DNPH) were well separated in less than 15 min under the optimized chromatographic conditions. The high separation speed, good selectivity and perfect column efficiency are obtained with the novel core-shell chromatographic stationary.

Key words: carbonyl compounds, core-shell chromatographic stationary phase, evaluation and application, high performance liquid chromatography (HPLC)

中图分类号: