色谱

色谱 ›› 2010, Vol. 28 ›› Issue (09): 889-892.DOI: 10.3724/SP.J.1123.2010.00889

• 研究论文 • 上一篇    下一篇

阴离子耗尽进样胶束扫集毛细管电动色谱法在线富集测定甘草黄酮类化合物

陈新1, 袁红萍2, 曹玉华1*, 陈秋云1   

  1. 1. 江南大学化学与材料工程学院, 江苏 无锡 214122; 2. 常州纺织服装职业技术学院纺织化学工程系, 江苏 常州 213164
  • 收稿日期:2010-05-27 修回日期:2010-07-29 出版日期:2010-09-28 发布日期:2010-09-28
  • 通讯作者: 曹玉华,博士,教授,主要研究方向为毛细管电泳.
  • 基金资助:

    国家自然科学基金项目(No. 20775028)和江南大学青年基金项目(No. 20070337).

On-line sample stacking for the analysis of glycyrrhiza flavonoids by anion selective exhaustive injection-sweeping micellar electrokinetic chromatography

CHEN Xin1, YUAN Hongping2, CAO Yuhua1*, CHEN Qiuyun1   

  1. 1. School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China; 2. Department of Textile and Chemical Engineering, Changzhou Textile and Garment Institute, Changzhou 213164, China
  • Received:2010-05-27 Revised:2010-07-29 Online:2010-09-28 Published:2010-09-28

PDF

385

被引次数

40

摘要： 建立了一个毛细管胶束电动色谱(MEKC)在线富集阴离子耗尽进样(ASEI)联用扫集(sweeping)技术测定3种甘草黄酮化合物(异甘草素、甘草素和甘草苷)的方法。考察了MEKC的分离条件和富集体系的优化条件,其中样品基质、水塞进入时间、进样时间对目标化合物的富集效果有较大的影响。在优化实验条件下,异甘草素、甘草素和甘草苷的富集倍数分别提高了110、120、300倍,检出限分别为0.015、0.014、0.011mg/L。该方法用于中药制剂中甘草素、异甘草素和甘草苷含量的测定,回收率在90.6%～107%之间,相对标准偏差(RSD)均小于4.5%(n=3)。实验证明,该方法可成功地应用于实际样品中甘草素、异甘草素和甘草苷含量的测定

关键词: 甘草黄酮, 毛细管胶束电动色谱, 扫集法, 阴离子耗尽进样

Abstract: An on-line preconcentration technique which coupled anion selective exhaustive injection (ASEI)-sweeping technology with micellar electrokinetic chromatography (MEKC) was used to analyze three glycyrrhiza flavonoids (isoliquiritigenin, liquiritigenin and liquiritin) in medicine. The separation conditions were optimized in conventional MEKC. The sample matrix, injection time of water and injection time of sample were found to be the predominant influences on the glycyrrhiza flavonoids stacking. Under optimal conditions, the detection sensitivities were improved by 110, 120, 300 times for isoliquiritigenin, liquiritigenin and liquiritin, respectively. The limits of detection (LODs) were 0.015, 0.014, 0.011mg/L for isoliquiritigenin, liquiritigenin and liquiritin, respectively. The recoveries were ranged in 90.6%~107% and relative standard deviations (RSDs) were less than 4.5%(n=3). This method was successfully applied for the detection of isoliquiritigenin, liquiritigenin and liquiritin in real samples.

Key words: anion selective exhaustive injection (ASEI), glycyrrhiza flavonoids, sweeping, micellar electrokinetic chromatography (MEKC)