色谱

色谱 ›› 2011, Vol. 29 ›› Issue (02): 187-190.DOI: 10.3724/SP.J.1123.2011.00187

• 技术与应用 • 上一篇    

离子色谱法定量检测酒曲发酵液中的河豚毒素

舒静, 李柏林, 欧杰*   

  1. 上海海洋大学食品学院, 上海 201306
  • 收稿日期:2010-10-22 修回日期:2010-11-29 出版日期:2011-02-28 发布日期:2007-03-25
  • 通讯作者: 欧杰,硕士,副教授,研究方向为食品生物技术.
  • 基金资助:
    离子色谱;河豚毒素;酒曲

Determination of tetrodotoxin in fermentation broth of distiller’s yeast by ion chromatography

SHU Jing, LI Bailin, OU Jie*   

  1. College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China
  • Received:2010-10-22 Revised:2010-11-29 Online:2011-02-28 Published:2007-03-25
  • Supported by:
    ion chromatography （IC）; tetrodotoxin; distiller’s yeast

PDF

715

被引次数

20

摘要： 建立了一种离子色谱定量检测酒曲发酵液中河豚毒素的分析方法。样品经乙腈(含0.1%磷酸)溶液提取和阳离子交换柱净化后,采用离子交换色谱柱分离和紫外检测。在优化的条件下,酒曲样品中的河豚毒素在10～100 mg/L内呈良好的线性关系(r2=0.997),加标回收率为90%～103%,相对标准偏差小于4.9%,检出限(信噪比为3)为1.0 mg/L。结果表明,该方法能达到定量检测的目的。将该方法应用于实际样品的检测,验证了方法的可靠性。河豚毒素初步降解实验发现,随着时间的推移,酒曲中河豚毒素的含量逐渐减少,表明酒曲发酵液对河豚毒素的降解效果显著。

关键词: 河豚毒素, 酒曲, 离子色谱

Abstract: A method was developed for the quantitative analysis of tetrodotoxin (TTX) in fermentation broth of distiller’s yeast by ion chromatography. After extraction with acetonitrile solution (containing 0.1% phosphoric acid) and purification with an ion-exchange column, the tetrodotoxin was separated by ion chromatography and detected by a ultraviolet-visible (UV-VIS) absorbance detector. The experimental results showed that the tetrodotoxin had a good linearity (r2=0.997) in the range of 10~100 mg/L and the detection limit (3 of signal-to-noise ratio) was 1.0 mg/L. The average recoveries were between 90%~103% with a relative standard deviation lower than 4.9%. The analysis of real samples verified the reliability of this method and demonstrated that the ion chromatography can be used for the quantification detection of the tetrodotoxin. The degradation experiment results suggested that distiller’s yeast had a remarkable effect on the tetrodotoxin degradation.

Key words: ion chromatography, tetrodotoxin, distiller's yeast