色谱

色谱 ›› 2011, Vol. 29 ›› Issue (02): 152-156.DOI: 10.3724/SP.J.1123.2011.00152

• 研究论文 • 上一篇    下一篇

他汀类药物在微乳液相色谱体系中的保留行为

施介华1,2*, 薛竹1   

  1. 1. 浙江工业大学药学院, 浙江 杭州 310032; 2. 浙江工业大学 绿色化学合成技术国家重点实验室培育基地, 浙江 杭州 310032
  • 收稿日期:2010-11-07 修回日期:2010-12-06 出版日期:2011-02-28 发布日期:2007-03-25
  • 通讯作者: 施介华,博士,教授,主要从事药物分析和药物合成研究.

Retention behavior of statins in microemulsion liquid chromatography

SHI Jiehua1,2*, XUE Zhu1   

  1. 1. College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310032, China;2. State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, Zhejiang University of Technology, Hangzhou 310032, China
  • Received:2010-11-07 Revised:2010-12-06 Online:2011-02-28 Published:2007-03-25

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514

被引次数

5

摘要： 以普伐他汀钠、阿托伐他汀钙、辛伐他汀和洛伐他汀为研究对象,考察了微乳液流动相中表面活性剂的浓度、油相浓度、助表面活性剂浓度以及流动相的pH值等对他汀类药物在微乳液相色谱体系中保留行为的影响。实验结果表明,微乳流动相中表面活性剂、助表面活性剂、亲脂性溶剂的浓度对他汀类药物保留行为的影响与理论模型一致;流动相的pH值对酸性他汀类药物保留行为的影响与理论模型基本一致,对中性他汀类药物保留行为的影响存在着隐函数关系。所建立的保留模型能较好地反映微乳液组成对他汀类药物保留行为的影响。

关键词: 保留模型, 保留行为, 他汀类药物, 微乳液相色谱

Abstract: The effects of the concentration of surfactant, co-surfactant and lipophilic organic solvent and the pH value of mobile phase on the retention behavior of statins in microemulsion liquid chromatography have been investigated using pravastatin sodium, atorvastatin calcium, lovastatin and simvastatin as model compounds. The experimental results showed that the effects of the concentration of surfactant, co-surfactant and lipophilic organic solvent on the retention behavior of statins in microemulsion liquid chromatography were fully consistent with the theoretical modeling. The effect of the pH value of the mobile phase on the retention behavior of acid statins was fully consistent with the theoretical modeling. The relationship between the retention factor of neutral statins and the concentration of hydrogen ion was an implicit function. The retention modeling can reflect the effect of composition of microemulsion solution on the retention behavior of statins drugs.

Key words: microemulsion liquid chromatography （MELC）, retention behavior, retention modeling, statins