色谱

色谱 ›› 2011, Vol. 29 ›› Issue (05): 458-461.DOI: 10.3724/SP.J.1123.2011.00458

• 技术与应用 • 上一篇    下一篇

高效液相色谱法同时测定化妆品中的3种苯扎氯铵同系物

刘艳琴, 王浩*, 杨红梅, 史海良, 郭启雷   

  1. 北京市海淀区产品质量监督检验所, 北京 100094
  • 收稿日期:2011-01-17 修回日期:2011-02-26 出版日期:2011-05-28 发布日期:2011-05-28
  • 通讯作者: 王浩,硕士,高级工程师,主要从事色谱检测方面研究. Tel: (010)82479365,
  • 基金资助:
    国家质量监督检验总局质量检验公益性行业科研专项项目(200910267-02).

Simultaneous determination of 3 benzalkonium chloride homologsin cosmetics by high performance liquid chromatography

LIU Yanqin, WANG Hao*, YANG Hongmei, SHI Hailiang, GUO Qilei   

  1. Haidian District Institute of Products Quality Supervision and Inspection, Beijing 100094, China
  • Received:2011-01-17 Revised:2011-02-26 Online:2011-05-28 Published:2011-05-28

PDF

729

被引次数

35

摘要： 建立了采用高效液相色谱-二极管阵列检测器(HPLC-DAD)同时检测化妆品中3种苯扎氯铵同系物(n-C12H25-C9H13NCl、n-C14H29-C9H13NCl、n-C16H33-C9H13NCl)的方法。采用含0.5%甲酸的甲醇超声提取样品,以CAPCELL PAK SCX色谱柱(250 mm×4.6 mm, 5 μm)分离,流动相为40 mmol/L乙酸铵水溶液(含0.1%三乙胺,pH 4.0)和乙腈,梯度洗脱,流速1.0 mL/min,检测波长260 nm,柱温25 ℃,进样量20 μL。该方法的检出限50.0 mg/kg,定量限200.0 mg/kg,线性范围5.0～3000.0 mg/L,加标回收率92.5%～102.1%,相对标准偏差为3.81%～6.66%。结果表明,该方法快速、准确,能够同时测定化妆品中3种苯扎氯铵同系物。

关键词: 苯扎氯铵同系物, 高效液相色谱法, 化妆品

Abstract: An analytical method based on high performance liquid chromatography with a diode array detector (HPLC-DAD) has been established for the simultaneous determination of 3 benzalkonium chloride homologs (n-C12H25-C9H13NCl, n-C14H29-C9H13NCl, n-C16H33-C9H13NCl) in cosmetics. The sample was extracted with methanol (including 0.5% formic acid) under ultrasonic operation, the HPLC separation was carried out on a CAPCELL PAK SCX column (250 mm×4.6 mm, 5 μm), the mobile phases were 40 mmol/L ammonium acetate solution (including 0.1% triethylamine) and acetonitrile with gradient elution, the flow rate was 1.0 mL/min, the detection wavelength was 260 nm, the column temperature was 25 ℃, and the injection volume was 20 μL. The limit of detection was 50.0 mg/kg and the quantitation limit was 200.0 mg/kg for 3 benzalkonium chloride homologs in cosmetics. The linear plots were obtained between 5.0 mg/L and 3000.0 mg/L. Overall recoveries were between 92.5% and 102.1% with the relative standard deviations (RSDs) between 3.81% and 6.66%. The method is simple, rapid, accurate and suitable for the determination of 3 benzalkonium chloride homologs in cosmetics.

Key words: benzalkonium chloride homologs, cosmetics, high performance liquid chromatography (HPLC)