色谱

色谱 ›› 2011, Vol. 29 ›› Issue (05): 439-442.DOI: 10.3724/SP.J.1123.2011.00439

• 研究论文 • 上一篇    下一篇

反相高效液相色谱法同时测定咖啡豆中的6种酚酸类化合物

龙文静1, 张盛1,3*, 袁玲2, 李银花1,2, 刘仲华1,2*   

  1. 1. 国家植物功能成分利用工程技术研究中心, 湖南 长沙 410128; 2. 湖南农业大学 茶学教育部重点实验室, 湖南 长沙 410128; 3. 湖南农业大学园艺园林学院植物资源工程系, 湖南 长沙 410128
  • 收稿日期:2011-01-05 修回日期:2011-03-18 出版日期:2011-05-28 发布日期:2011-05-28
  • 通讯作者: 张盛,博士,副教授,主要研究方向为植物功能成分化学.刘仲华,博士生导师,教授,主要研究方向为植物功能成分化学.
  • 基金资助:
    教育部创新团队计划(No. 20091101).

Simultaneous determination of 6 phenolic acids in coffee beans by reversed-phase high performance liquid chromatography

LONG Wenjing1, ZHANG Sheng1,3*, YUAN Ling2, LI Yinhua1,2, LIU Zhonghua1,2*   

  1. 1. National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Changsha 410128, China; 2. Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China; 3. Department of Botanical Resources Engineering, College of Horticulture & Gardening, Hunan Agricultural University, Changsha 410128, China
  • Received:2011-01-05 Revised:2011-03-18 Online:2011-05-28 Published:2011-05-28

PDF

589

被引次数

20

摘要： 建立了同时测定咖啡豆中6种酚酸类化合物(咖啡酸、3-咖啡酰奎尼酸、4-咖啡酰奎尼酸、5-咖啡酰奎尼酸、3,5-二咖啡酰奎尼酸、4,5-二咖啡酰奎尼酸)的反相高效液相色谱测定方法。采用Kromasil C18柱(200 mm×4.6 mm, 5 μm),以乙腈和0.1%甲酸水溶液为流动相进行梯度洗脱,二极管阵列检测器检测,45 min内可对6种目标物进行同时检测,且各化合物都能达到基线分离。经测定,样品中6种酚酸类化合物的加标回收率为90.76%～104.73%,相对标准偏差为0.7%～3.9%。该法简便、快速、灵敏度高,适用于咖啡豆中6种酚酸类化合物的同时分析以及咖啡豆原料与制品的质量控制和综合评价。

关键词: 反相高效液相色谱法, 酚酸类化合物, 咖啡豆

Abstract: Abstract：A method of reversed-phase high performance liquid chromatography coupled with diode array detector ( HPLC-DAD ) was established for the simultaneous determination of 6 phenolic acids ( Caffeic acid，3-O-Caffeoylquinic acid, 4-O-Caffeoylquinic acid, 5-O-Caffeoylquinic acid, 3,5-O-Dicaffeoylquinic acid, 4,5-O-Dicaffeoylquinic acid ) in green coffee beans. Separation was achieved using a Kromasil C18 column (4.6 mm × 200 mm, 5 μm ), under the gradient elution with the mobile phase of acetonitrile and 0.1% ( v/v ) formic acid / water. The 6 phenolic acids were well separated within 45 min. The recoveries from 91.52% to 103.26% with the relative standard deviation between 0.55% ~1.83%. The limits of detection were in the range of 0.01~ 0.15 μg. The method is simple, rapid and highly sensitive, suitable for the simultaneous determination of 6 phenolic acids and quality control of coffee beans.

Key words: coffee bean, phenolic acids, reversed-phase high performance liquid chromatography(RP-HPLC)