色谱

色谱

• 研究论文 • 上一篇    下一篇

超声波萃取-高效液相色谱测定咖啡粉和速溶咖啡中的葫芦巴碱

刘宏程, 黎其万*, 邵金良, 严红梅, 兰珊珊   

  1. 云南省农业科学院质量标准与检测技术研究所, 云南 昆明 650223
  • 收稿日期:2011-06-13 修回日期:2011-07-20 出版日期:2011-11-28 发布日期:2011-12-25
  • 通讯作者: 黎其万,研究员,研究方向为农产品安全.
  • 基金资助:

    云南咖啡产业技术标准体系建设基金项目(2010-009)和云南省地方标准建立基金项目.

Determination of trigonelline in coffee powder and instant coffee by ultrasonic extraction and high performance liquid chromatography

LIU Hongcheng, LI Qiwan*, SHAO Jinliang, YAN Hongmei, LAN Shanshan   

  1. Institute of Quality Standard and Testing Technology, Yunnan Academy of Agricultural Sciences, Kunming 650223, China
  • Received:2011-06-13 Revised:2011-07-20 Online:2011-11-28 Published:2011-12-25

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摘要： 建立了高效液相色谱测定咖啡粉和速溶咖啡中葫芦巴碱含量的方法。采用Waters BondPak NH2色谱柱(250 mm×4.6 mm, 5 μm),以甲醇-水(82:18, v/v)为流动相,流速0.8 mL/min,检测波长260 nm。葫芦巴碱线性范围为1～40 mg/L (相关系数为0.9998)。分别进行日内、日间和人员比对测定葫芦巴碱的重复性试验及加标回收率试验。经测定,样品的加标回收率大于90%,相对标准偏差小于3%。比较了超声波萃取和热浸提萃取方法测定葫芦巴碱含量的差异,两个方法的测定结果符合线性关系,相关系数为0.9964。该法简便、快速、灵敏度高,适用于咖啡豆中葫芦巴碱分析以及咖啡豆原料与制品的质量控制。

关键词: 超声波萃取, 高效液相色谱, 葫芦巴碱, 咖啡粉, 速溶咖啡

Abstract: A method of high performance liquid chromatography was established for the analysis of trigonelline in coffee powder and instant coffee. The separation was performed on a BondPak NH2 column (250 mm×4.6 mm, 5 μm). The mobile phase was methanol-water (82:18, v/v) at a flow rate of 0.8 mL/min. The detection wavelength was set at 260 nm. The method had good linearity in the range of 1~40 mg/L (the correlation coefficient was 0.9998). The repeatability of the method was performed at one day, different days, and by two analysts. The accuracy of the method was evaluated by recovery study. The recoveries were more than 90% with the relative standard deviations less than 3%. The contents of trigonelline in samples were assessed with ultrasonic extraction and hot water extraction, separately, and the regression coefficient between trigonelline contents obtained under the two extraction methods was 0.9964. The method is simple, rapid, highly sensitive, and suitable for the analysis of trigonelline and quality control of coffee samples.

Key words: coffee powder, high performance liquid chromatography (HPLC), instant coffee, trigonelline, ultrasonic extraction