通过柱切换技术与高效液相色谱联用的限进性柱对盐酸贝那普利的在线富集性能

doi:10.3724/SP.J.1123.2012.11021

色谱 ›› 2013, Vol. 31 ›› Issue (5): 451-455.DOI: 10.3724/SP.J.1123.2012.11021

通过柱切换技术与高效液相色谱联用的限进性柱对盐酸贝那普利的在线富集性能

张晓惠¹, 王荣^1,2*, 谢华¹, 尹强¹, 李晓云¹, 贾正平^1,2*, 武晓玉¹, 张娟红¹, 李文斌¹

1. 兰州军区兰州总医院全军高原损伤防治重点实验室, 甘肃兰州 730050;
2. 兰州大学药学院, 甘肃兰州 730050

收稿日期:2012-11-28 修回日期:2013-01-14 出版日期:2013-05-28 发布日期:2013-05-16
通讯作者: 王荣,Tel:(0931)8994675,E-mail:wangrong-69@163.com;贾正平,E-mail:Jiazp166@yahoo.com.
基金资助:
国家科技部重大资助项目(2008ZXJ09014-010);全军"十二五"医药科研重点项目(BWS12J012);全军"十二五"医药科研面上项目(CWS11C231).

Online enrichment ability of restricted-access column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride

ZHANG Xiaohui¹, WANG Rong^1,2*, XIE Hua¹, YIN Qiang¹, LI Xiaoyun¹, JIA Zhengping^1,2*, WU Xiaoyu¹, ZHANG Juanhong¹, LI Wenbin¹

1. PLA Key Laboratory of the Plateau of the Environmental Damage Control, Lanzhou General Hospital, Lanzhou Command, Lanzhou 730050, China;
2. School of Pharmacy, Lanzhou University, Lanzhou 730050, China

Received:2012-11-28 Revised:2013-01-14 Online:2013-05-28 Published:2013-05-16

摘要/Abstract

摘要：

以自制的限进性填料柱为预处理富集柱,Luna C₁₈柱为分析柱,通过柱切换技术将限进性填料柱与高效液相色谱联用(RAM-HPLC),研究了盐酸贝那普利的在线富集效果。考察了进样体积与峰面积、系统总压力的关系,以及常规进样与大体积进样的差别。当进样体积在100 μL以内时,峰面积随进样体积的增加而增加;当进样体积大于80 μL时,系统总压力变化明显。考虑对整个系统的保护,选择80 μL作为最大进样体积。同一浓度的样品进样20 μL与进样80 μL所得峰面积之间的线性关系良好。RAM柱对盐酸贝那普利具有良好的富集作用,能够有效提高HPLC的灵敏度,而且具有简单、经济的特点。

关键词: 高效液相色谱, 限进性填料, 血样, 盐酸贝那普利, 在线富集, 直接进样

Abstract:

The online enrichment ability of the restricted-access media (RAM) column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride in plasma was studied. The RAM-HPLC system consisted of an RAM column as enrichment column and a C18 column as analytical column coupled via the column switching technique. The effects of the injection volume on the peak area and the systematic pressure were studied. When the injection volume was less than 100 μL, the peak area increased with the increase of the injection volume. However, when the injection volume was more than 80 μL, the pressure of whole system increased obviously. In order to protect the whole system, 80 μL was chosen as the maximum injection volume. The peak areas of ordinary injection and the large volume injection showed a good linear relationship. The enrichment ability of RAM-HPLC system was satisfactory. The system was successfully used for the separation and detection of the trace benazepril hydrochloride in rat plasma after its administration. The sensitivity of HPLC can be improved by RAM pre-enrichment. It is a simple and economic measurement method.

Key words: benazepril hydrochloride, direct injection, high-performance liquid chromatography (HPLC), online enrichment, restricted-access media (RAM), serum

中图分类号:

O658

张晓惠, 王荣, 谢华, 尹强, 李晓云, 贾正平, 武晓玉, 张娟红, 李文斌. 通过柱切换技术与高效液相色谱联用的限进性柱对盐酸贝那普利的在线富集性能[J]. 色谱, 2013, 31(5): 451-455.

ZHANG Xiaohui, WANG Rong, XIE Hua, YIN Qiang, LI Xiaoyun, JIA Zhengping, WU Xiaoyu, ZHANG Juanhong, LI Wenbin. Online enrichment ability of restricted-access column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride[J]. Chinese Journal of Chromatography, 2013, 31(5): 451-455.

参考文献

[1] Hu X G, Luo Y, Zhou Q X, et al. Chinese Journal of Analytical Chemistry (胡献刚, 罗义, 周启星, 等. 分析化学), 2008, 36(9): 1162
[2] Xu W J, Su S F, Jiang P, et al. J Chromatogr A, 2010, 1217: 7198
[3] Gil G, Brennan J, Throckmorton D J, et al. J Chromatogr B, 2011, 879: 1112
[4] Wang Y, Wang Y X, Chen L, et al. J Magn Magn Mater, 2012, 324: 410
[5] Kishida K, Furusawa N. J Chromatogr A, 2004, 1028(1): 175
[6] Lamprecht G, Stoschitzky K. J Chromatogr B, 2009, 877(29): 3489
[7] Guo Z Q, Ma J, Jia Z P, et al. Chinese Journal of Pharmaceutical Analysis (郭志强, 马骏, 贾正平, 等. 药物分析杂志), 2006, 26(6): 758
[8] Gasparrini F, Cancelliere G, Ciogli A, et al. J Chromatogr A, 2008, 1191: 205
[9] Fan P C, Jia Z P, Ma J, et al. Chinese Journal of Pharmaceutical Analysis (樊鹏程, 贾正平, 马骏, 等. 药物分析杂志), 2008, 28(2): 329
[10] Yang P, Wang R, Ma J, et al. Analytical Instrumentation (杨沛, 王荣, 马骏, 等. 分析仪器), 2011(3): 1
[11] Guo Z Q, Ma J, Jia Z P, et al. Chinese Journal of Pharmaceutical Analysis (郭志强, 马骏, 贾正平, 等. 药物分析杂志), 2006, 26(8): 1071
[12] Ma J, Jia Z P, Wang R, et al. Chinese Journal of Pharmaceutical Analysis (马骏, 贾正平, 王荣, 等. 药物分析杂志), 2005, 25(12): 1420
[13] Wu X Y, Wang R, Xie H, et al. Chinese Journal of Chromatography (武晓玉, 王荣, 谢华, 等. 色谱), 2011, 29(12): 1205
[14] Hilton J, Yokoi F, Dannals R F, et al. Nucl Med Biol, 2000, 27(6): 627
[15] Wang X D, Chan E, Chen X, et al. J Pharm Biomed Anal, 2007, 44: 224
[16] Wang R, Jia Z P, Xie H, et al. China Patent (王荣, 贾正平, 谢华, 等. 中国专利), 2012100351314. 2012-02-16
[17] Wu X Y, Wang R, Xie H, et al. Chinese Journal of Chromatography (武晓玉, 王荣, 谢华, 等. 色谱), 2012, 30(8): 810

通过柱切换技术与高效液相色谱联用的限进性柱对盐酸贝那普利的在线富集性能

Online enrichment ability of restricted-access column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

扫码分享

参考文献

相关文章 15

编辑推荐

Metrics

[1]	史洪飞, 徐伯芃, 徐成鑫, 周修齐, 许宏福. 溶剂萃取-高效液相色谱-四极杆飞行时间质谱法快速提取检测干燥恰特草中5种生物碱成分[J]. 色谱, 2023, 41(9): 771-780.
[2]	赵芮, 黄晴雯, 余智颖, 韩铮, 范楷, 赵志辉, 聂冬霞. QuEChERS-超高效液相色谱-串联质谱法同时测定水果中36种真菌毒素[J]. 色谱, 2023, 41(9): 760-770.
[3]	刘宇, 邢家溧, 沈坚, 毕晓丽, 毛玲燕, 徐晓蓉, 张书芬, 娄永江, 吴希, 穆应花. 高效液相色谱-蒸发光散射检测法同时测定固态食品中6种稀有糖[J]. 色谱, 2023, 41(9): 781-788.
[4]	岳超, 赵超群, 毛思浩, 王展华, 施贝, 徐欣丰, 梁晶晶. 通过式固相萃取净化-超高效液相色谱-串联质谱法测定液体乳中10种氨基甲酸酯类农药残留[J]. 色谱, 2023, 41(9): 807-813.
[5]	钱正明, 吴梦奇, 谭国英, 金李玲, 李宁, 谢菊英. 高效液相色谱紫外等吸收波长法快速测定秦皮中秦皮甲素和秦皮乙素[J]. 色谱, 2023, 41(8): 690-697.
[6]	吴萍萍, 林仁义, 黄丽英. 三相中空纤维液相微萃取-高效液相色谱法测定铁皮石斛和金线莲中3种植物生长调节剂[J]. 色谱, 2023, 41(8): 683-689.
[7]	马超, 倪洪星, 戚羽霖. 超高效液相色谱-傅里叶变换离子回旋共振质谱法解析溶解性有机质的化学多样性[J]. 色谱, 2023, 41(8): 662-672.
[8]	孟二琼, 念琪循, 李峰, 张秋萍, 许茜, 王春民. 磺酸化磁性氮化碳固相萃取-超高液相色谱-串联质谱筛检淡水鱼中孔雀石绿和隐色孔雀石绿[J]. 色谱, 2023, 41(8): 673-682.
[9]	曹沁玲, 赵小丹, 沈国滨, 汪竹琴, 章弘扬, 张敏, 胡坪. 基于超高效液相色谱-电雾式检测的槐糖脂指纹图谱[J]. 色谱, 2023, 41(8): 722-729.
[10]	陈东洋, 张昊, 张磊, 王一红, 王小丹, 冯家力, 梁静, 钟旋. 固相萃取-超高效液相色谱-串联质谱法测定发酵食品中的曲酸[J]. 色谱, 2023, 41(7): 632-639.
[11]	王秋旭, 冯启言, 朱雪强. 加速溶剂萃取-固相萃取净化结合超高效液相色谱-串联质谱法测定沉积物中双酚类化合物[J]. 色谱, 2023, 41(7): 582-590.
[12]	杨哲, 吕建霞, 吴一荻, 蒋力维, 李冬梅. 超高效液相色谱法同时测定电子烟油中的5种吲哚/吲唑酰胺类合成大麻素[J]. 色谱, 2023, 41(7): 602-609.
[13]	夏宝林, 汪仕韬, 殷晶晶, 张维益, 杨娜, 刘强, 吴海晶. 自动上样固相萃取-超高效液相色谱-串联质谱法同时测定水中9类43种抗菌药物残留[J]. 色谱, 2023, 41(7): 591-601.
[14]	童学智, 陈东洋, 冯家力, 范翔, 张昊, 杨胜园. QuEChERS-超高效液相色谱-串联质谱法快速测定水产品中5种卤代苯醌[J]. 色谱, 2023, 41(6): 490-496.
[15]	王园媛, 李璐璐, 吕佳, 陈永艳, 张岚. 固相萃取-超高效液相色谱-三重四极杆质谱法测定饮用水中13种卤代苯醌类消毒副产物[J]. 色谱, 2023, 41(6): 482-489.