不同产地龙葵药材的高效液相色谱-蒸发光散射检测指纹图谱

doi:10.3724/SP.J.1123.2015.04022

色谱 ›› 2015, Vol. 33 ›› Issue (8): 809-815.DOI: 10.3724/SP.J.1123.2015.04022

• 特别策划:第20届全国色谱学术报告会专栏 • 上一篇下一篇

不同产地龙葵药材的高效液相色谱-蒸发光散射检测指纹图谱

王珏¹, 金一宝¹, 王铁杰¹, 李晓帆²

1. 深圳市药品检验所, 深圳药品质量标准研究重点实验室, 广东深圳 518057;
2. 深圳大学, 深圳市微生物基因工程重点实验室, 广东深圳 518060

收稿日期:2015-04-14 出版日期:2015-08-08 发布日期:2015-07-27
通讯作者: 李晓帆
基金资助:
2015年广东省食品药品监督管理局食品药品检验检测技术创新专项资金资助项目(2015ZX06).

Fingerprint of Solanum nigrum L. from different habitats using high performance liquid chromatography with evaporative light-scattering detection

WANG Jue¹, JIN Yibao¹, WANG Tiejie¹, LI Xiaofan²

1. Shenzhen Institute for Drug Control, Shenzhen Key Laboratory of Drug Quality Standard Research, Shenzhen 518057, China;
2. Shenzhen University, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen 518060, China

Received:2015-04-14 Online:2015-08-08 Published:2015-07-27

摘要/Abstract

摘要：

采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)来研究不同产地龙葵药材品质的差异,为龙葵药材质量标准的制定提供参考依据。使用Phenomenex C₁₈色谱柱,流动相为乙腈-20%(v/v)甲醇水溶液(含0.03%(v/v)三乙胺),梯度洗脱,蒸发管温度为40 ℃。利用"中药色谱指纹图谱相似度评价系统"对所采集的色谱图进行相似度比对,选择相似度在0.9~1.0之间的色谱图归一化拟合成龙葵的标准指纹图谱,并利用对照品进行色谱峰指认。研究结果表明,不同产地来源的龙葵药材之间具有显著的差异,依据HPLC-ELSD采集到的非挥发类成分可对中药材进行区分。该方法为龙葵药材的真实性、优良性和稳定性评价提供了一种新的、具有广阔应用前景的技术手段。

关键词: 高效液相色谱法, 龙葵, 蒸发光散射检测, 指纹图谱

Abstract:

Solanum nigrum L. contains a variety of active ingredients. It has been applied broadly in clinical practice. However, the compositions of Solanum nigrum L. from different cultivated areas are quite different. Therefore, it is necessary to set up a standard fingerprint. In this work, a series of Solanum nigrum L. samples were collected from different habitats. A high performance liquid chromatography with evaporative light-scattering detection (HPLC-ELSD) method was developed to study the differences in quality of these samples. The method was optimized by comparing different columns, gradient or isocratic elution and temperatures of evaporating tube. A Phenomenex C₁₈ column was finally selected for separation with gradient elution. The mobile phases were acetonitrile-20% (v/v) methanol aqueous solution containing 0.03% (v/v) triethylamine. The temperature of evaporating tube was set at 40 ℃. The validation was performed and the results showed that the method had good precision, repeatability and stability. The validated method was applied to detect Solanum nigrum L. from different habitats. The similarities of the acquired chromatograms were analyzed by using the similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (TCM). The chromatograms with a similarity between 0.9 and 1.0 were normalized and fitted to form a standard fingerprint of Solanum nigrum L. Thirteen common peaks were found and eleven peaks were identified with authentic standards. The results showed that remarkable differences were found among Solanum nigrum L. from different habitats. These herbs could be distinguished based on the compositions detected by HPLC-ELSD. This method provided a technology to evaluate the homogeneity and stability of Solanum nigrum L with wide application prospects and practical value.

Key words: Solanum nigrum L., evaporative light-scattering detection (ELSD), fingerprints, high performance liquid chromatography (HPLC)

中图分类号:

O658

王珏, 金一宝, 王铁杰, 李晓帆. 不同产地龙葵药材的高效液相色谱-蒸发光散射检测指纹图谱[J]. 色谱, 2015, 33(8): 809-815.

WANG Jue, JIN Yibao, WANG Tiejie, LI Xiaofan. Fingerprint of Solanum nigrum L. from different habitats using high performance liquid chromatography with evaporative light-scattering detection[J]. Chinese Journal of Chromatography, 2015, 33(8): 809-815.

参考文献

[1] Pharmacopoeia Commission of People's Republic of China. Pharmacopoeia of People's Republic of China, Part 1. Beijing: China Medical Science Press (国家药典委员会. 中华人民共和国药典, 一部. 北京: 中国医药科技出版社), 2000: Appendix 23

[2] Li Z H, Meng J H, Xiao H. Traditional Chinese Drug Research & Clinical Pharmacology (李志辉, 孟军华, 肖晖. 中药新药与临床药理), 2012, 23(6): 661

[3] Liu L F, Gao B Y, Gao S H. World Journal of Integrated Traditional and Western Medicine (刘林凤, 高宝益, 高淑红. 世界中西医结合杂志), 2013, 8(2): 144

[4] Yuan H J, Chen Y G, Cai B C, et al. China Journal of Chinese Materia Medica (袁海建, 陈宜刚, 蔡宝昌, 等. 中国中药杂志), 2011, 36(12): 1630

[5] Shan H J, Wang B. Chinese Journal of Experimental Traditional Medical Formulae (单会娇, 王冰. 中国实验方剂学杂志), 2010, 16(9): 100

[6] Luo W J, Wang G H, Zhou X L, et al. Modern Oncology (罗文娟, 王光辉, 周新兰, 等. 现代肿瘤医学), 2007, 15(3): 307

[7] Loganayaki N, Siddhuraju P, Manian S. Food Sci Biotechnol, 2010, 19(1): 121

[8] Wang H C, Chung P J, Wu C H, et al. J Sci Food Agric, 2011, 91(1): 178

[9] Shan H J, Zhang J K, Xu L, et al. Chinese Traditional Patent Medicine (单会娇, 张建逵, 许亮, 等. 中成药), 2011, 33(3): 483

[10] WHO. Guidelines for the Assessment of Herbal Medicines. Geneva, 1996: 178

[11] Center for Drug Evaluation and Research (CDER) of Food and Drug Administration (FDA). Guidance for Industry-Botanical Drug Products (Draft Guidance). Rockville, 2004

[12] Sun G X, Dou X W, Yang L P, et al. Chinese Journal of Chromatography (孙国祥, 豆小文, 杨兰萍, 等. 色谱), 2013, 31(5): 456

[13] Zhou X L. [PhD Dissertation]. Shenyang: Shenyang Pharmaceutical University (周新兰. [博士学位论文]. 沈阳: 沈阳药科大学), 2006

[14] Luo D, Luo G A, Chen L, et al. Pharmacy and Clinics of Chinese Materia Medica (罗丹, 罗国安, 陈丽, 等. 中药与临床), 2014, 5(6): 21

[15] Ding H L, Li C, Jin P, et al. Chinese Journal of Chromatography (丁洪流, 李灿, 金萍, 等. 色谱), 2013, 31(8): 804

[16] Zhao H, Wang Y H, Liu F, et al. Chinese Journal of Chromatography (赵卉, 王玉红, 刘芳, 等. 色谱), 2013, 31(8): 795

不同产地龙葵药材的高效液相色谱-蒸发光散射检测指纹图谱

Fingerprint of Solanum nigrum L. from different habitats using high performance liquid chromatography with evaporative light-scattering detection

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