色谱 ›› 2016, Vol. 34 ›› Issue (12): 1131-1136.DOI: 10.3724/SP.J.1123.2016.08036

• 专论与综述 • 上一篇    下一篇

外泌体分离及其蛋白质组学分析的研究进展

翁叶靖1,2, 随志刚1, 张丽华1, 张玉奎1   

  1. 1. 中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 辽宁 大连 116023;
    2. 中国科学院大学, 北京 100049
  • 收稿日期:2016-08-30 出版日期:2016-12-08 发布日期:2013-04-24
  • 通讯作者: 张丽华,Tel:(0411)84379720,E-mail:lihuazhang@dicp.ac.cn
  • 基金资助:

    国家自然科学基金(21305140);国家重大科学仪器设备开发专项(2012YQ120044-8).

Recent advances on exosomes isolation methods and proteomic analysis

WENG Yejing1,2, SUI Zhigang1, ZHANG Lihua1, ZHANG Yukui1   

  1. 1. CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2016-08-30 Online:2016-12-08 Published:2013-04-24
  • Supported by:

    National Natural Science Foundation of China (No. 21305140); National Important Project on Science Instrument (No. 2012YQ120044-8).

摘要:

外泌体是一种由细胞分泌的膜性囊泡小体,直径通常为30~100 nm,密度为1.10~1.18 kg/L。外泌体广泛存在于各种体液中,可携带脂类、蛋白质、信使RNAs(mRNAs)、microRNAs(miRNAs)、非编码RNAs(ncRNAs)等多种重要的生物功能分子。许多证据表明,外泌体形成了一种特殊的细胞间信息传递系统,不仅影响细胞的生理状态,而且与多种疾病的发生与发展密切相关。该文简要综述了近年来在外泌体的分离和纯化方面取得的研究进展,并对蛋白质组学技术在外泌体分析中的应用进行了概述。此外,还对外泌体蛋白质组学研究的发展前景进行了展望。

关键词: 蛋白质组学, 定量分析, 分离纯化, 外泌体, 综述, 综述

Abstract:

Exosomes are membrane-bound vesicles secreted by cells with the diameter of 30-100 nm and the density of 1.10-1.18 kg/L. Exosomes exist widely in almost all kinds of biofluids and contain a series of biologically important molecules, such as lipids, proteins, mRNAs, miRNAs and ncRNAs. Emerging evidence shows that exosomes could establish an intercellular information transfer system, thereby affecting the physiological status of cells and involving in various pathological processes. Herein, the recent advances on exosome isolation and purification methods are reviewed. In the meantime, the proteomic analysis of exosomes is briefly introduced. Finally, the future of exosome proteomic research is discussed.

Key words: exosomes, isolation and purification, proteomic, quantitative analysis, review

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