色谱 ›› 2017, Vol. 35 ›› Issue (1): 121-128.DOI: 10.3724/SP.J.1123.2016.08045

• 专论与综述 • 上一篇    下一篇

在线二维液相色谱技术在中药研究中的应用进展

高雯, 宋慧鹏, 杨华, 李萍   

  1. 天然药物活性组分与药效国家重点实验室, 中国药科大学, 江苏 南京 210009
  • 收稿日期:2016-08-31 出版日期:2017-01-08 发布日期:2013-05-06
  • 通讯作者: 李萍,Tel:(025)83271379,E-mail:liping2004@126.com.
  • 基金资助:

    国家自然科学基金(81503241);高等学校博士学科点专项科研基金(20130096140001).

Application progress of on-line two-dimensional liquid-chromatography in the analysis of traditional Chinese medicines

GAO Wen, SONG Huipeng, YANG Hua, LI Ping   

  1. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China
  • Received:2016-08-31 Online:2017-01-08 Published:2013-05-06
  • Supported by:

    National Natural Science Foundation of China (No. 81503241); Specialized Research Fund for the Doctoral Program of Higher Education (No. 20130096140001).

摘要:

中药的组成复杂,其化学成分的表征和识别一直是中药研究的基础和关键。在线二维液相色谱是基于两种分离模式构建的色谱分析技术,主要包括中心切割二维液相色谱和全二维液相色谱两种模式,因二者具有更高的峰容量而在中药研究中备受青睐。该文对在线二维液相色谱技术的概念和特点进行了讨论,并对二维液相色谱在中药研究中的应用进行了综述,以期为该技术在中药质量控制、物质基础表征、活性成分筛选等研究方面提供一定参考。

关键词: 活性成分筛选, 全二维液相色谱, 中心切割二维液相色谱, 中药分析, 综述

Abstract:

Traditional Chinese medicine (TCM) is a complex system, containing hundreds of components. The chemical profiling of TCMs is an important task and still facing great challenges. In recent years, the on-line two-dimensional liquid-chromatography (2D-LC) methods, emerging and powerful separation techniques, offer new possibilities for the global analysis of complex TCM samples. Recent advances of 2D-LC, including heart-cutting two-dimensional liquid-chromatography (heart-cutting LC-LC) and comprehensive two-dimensional liquid-chromatography (comprehensive LC×LC), applied to TCMs analysis are reviewed. Design and application of 2D-LC combined with turbulent flow chromatography (TFC) or cell membrane chromatography (CMC) applied for TCM bioactive components screening are described.

Key words: bioactive components screening, comprehensive two-dimensional liquid-chromatography (comprehensive LC×, heart-cutting two-dimensional liquid-chromatography (heart-cutting LC-LC), LC), review, traditional Chinese medicine analysis

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