色谱 ›› 2017, Vol. 35 ›› Issue (1): 129-137.DOI: 10.3724/SP.J.1123.2016.09005

• 专论与综述 • 上一篇    下一篇

微流控芯片分选临床样品中循环肿瘤细胞的研究进展

包建民, 王丹丹, 李优鑫   

  1. 天津大学药物科学与技术学院, 天津化学化工协同创新中心, 天津市现代药物传递及功能化高效能转化重点实验室, 天津 300072
  • 收稿日期:2016-09-01 出版日期:2017-01-08 发布日期:2013-05-06
  • 通讯作者: 李优鑫,Tel:(022)27892820,E-mail:lyx@tju.edu.cn.
  • 基金资助:

    国家自然科学基金项目(21375093,21605112);高等学校博士学科点专项科研基金项目(20130032120081);天津市应用基础与前沿技术研究计划青-项目(15JCQNJC43200).

Application advances of microfluidic chips for sorting circulating tumor cells in clinical samples

BAO James Jianmin, WANG Dandan, LI Youxin   

  1. School of Pharmaceutical Science and Technology, Tianjin University, Collaborative Innovation Center of Tianjin Chemical Engineering, Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Tianjin 300072, China
  • Received:2016-09-01 Online:2017-01-08 Published:2013-05-06
  • Supported by:

    National Natural Science Foundation of China (Nos. 21375093, 21605112); Special Research Found for the Doctoral Program of Higher Education (No. 20130032120081); Youth Fund of the Science and Technology Committee of Tianjin Municipal Government (No. 15JCQNJC43200).

摘要:

癌症作为常见病正严重威胁着我国乃至全球居民的健康。循环肿瘤细胞(CTCs)是一类由癌变部位释放并进入血液中的癌细胞,其在癌症的早期诊断、个体化及肿瘤转移机制研究等方面的作用正逐渐被发现和认可,但由于血液中的CTCs含量极少,对其分选极具挑战。微流控芯片作为一种微型化、高通量、集成化平台,在CTCs研究中彰显了独特的优势,相关报道也越来越多。随着研究的深入,微流控芯片技术不再局限于基于模型样品的方法学开发,而是更注重于能否用于临床实际样品中CTCs的检测,但目前未见该角度的综述报道。为此,文章综述了近年来用于临床实际样品CTCs分析的微流控芯片分选技术,并探讨了微流控芯片用于CTCs分选的发展趋势。

关键词: 分选, 临床样品, 微流控芯片, 循环肿瘤细胞, 综述

Abstract:

Cancer is becoming a common disease threatening seriously to the human health. Circulating tumor cells (CTCs) is a kind of cancer cells released from tumors and circulates with bloodstream. It is gradually discovered of the potential value of CTCs in diagnosis of cancer in initial stage, individual treatment and research of metastasis mechanism. However, it is a very challenge task for sorting the extremely few CTCs in blood. Microfluidic chip, a miniaturized, high-throughput and integrated platform, shows the unique advantages in the study of CTCs. With the progress of the research, the technology is no longer limited to the development of sorting method based on model samples, but scientists pay more attention to the analysis of clinical samples. To the best of our knowledge, there has been no paper summing up the progress of microfluidic chip techniques applied to the analysis of clinical samples recently. This review focuses on those microfluidic techniques that have been applied to sorting CTCs from the blood of cancer patients. Meanwhile, we predict the trends of sorting CTCs in clinical samples using microfluidic chip technology.

Key words: circulating tumor cells (CTCs), clinical samples, microfluidic chip, review, sorting

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