色谱

色谱 ›› 2017, Vol. 35 ›› Issue (3): 302-307.DOI: 10.3724/SP.J.1123.2016.10022

• 研究论文 • 上一篇    下一篇

大孔树脂-高速逆流色谱分离纯化薇甘菊中的黄酮类化合物

耿姗1, 王娟强1, 席兴军2, 初侨2, 董跟来2, 马晓萌1, 张惠文3, 魏芸1,3   

  1. 1. 北京化工大学化工资源有效利用国家重点实验室, 北京 100029;
    2. 中国标准化研究院, 北京 100191;
    3. 北京化工大学理学院, 北京 100029
  • 收稿日期:2016-10-12 出版日期:2017-03-08 发布日期:2013-07-16
  • 通讯作者: 魏芸,Tel&Fax:+86-10-64442928,E-mail:weiyun@mail.buct.edu.cn.
  • 基金资助:

    National Natural Science Foundation of China (No. 21075007); Program for New Century Excellent Talents in University (No. NCET-11-0563); Beijing Nova Program Interdisciplinary Cooperation Project (No. Z161100004916045).

Separation and purification of flavonoids from Mikania micrantha by macroporous resin combined with high speed countercurrent chromatography

GENG Shan1, WANG Juanqiang1, XI Xingjun2, CHU Qiao2, DONG Genlai2, MA Xiaomeng1, ZHANG Huiwen3, WEI Yun1,3   

  1. 1. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China;
    2. China National Institute of Standardization, Beijing 100191, China;
    3. School of Sciences, Beijing University of Chemical Technology, Beijing 100029, China
  • Received:2016-10-12 Online:2017-03-08 Published:2013-07-16
  • Supported by:

    National Natural Science Foundation of China (No. 21075007); Program for New Century Excellent Talents in University (No. NCET-11-0563); Beijing Nova Program Interdisciplinary Cooperation Project (No. Z161100004916045).

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被引次数

1

摘要：

该文建立了大孔树脂-高速逆流色谱分离薇甘菊中黄酮类物质的方法。分离条件为:采用大孔树脂AB-8，洗脱液为50%（v/v）乙醇水溶液，高速逆流色谱溶剂体系为正丁醇-乙酸-水（4：1：5，v/v）。从薇甘菊中分离到4种黄酮类物质:槲皮素-3-O-芸香糖苷（纯度90.2%）、山奈酚-3-O-芸香糖苷（纯度98.55%）、木犀草苷（纯度98.33%）和紫云英苷（纯度99.23%）。建立的大孔树脂-高速逆流色谱方法简单、高效，可扩展应用于从其他植物中分离黄酮类物质。

关键词: 大孔树脂, 分离, 高速逆流色谱, 黄酮类物质, 薇甘菊

Abstract:

The present study established a method for the separation and purification of four flavonoids quercetin-3-O-rutinoside, kaempferol-3-O-rutinoside, luteoloside and astragalin from Mikania micrantha by using macroporous resin combined with high speed countercurrent chromatography (HSCCC). Macroporous resin was AB-8 and the eluent was 50% (v/v) ethanol. A two-phase solvent system composed of n-butanol/acetic acid/water (4:1:5, v/v) was used in HSCCC. Quercetin-3-O-rutinoside, kaempferol-3-O-rutinoside, luteoloside and astragalin with the purity of 90.12%, 98.55%, 98.33% and 99.23%, respectively, were successfully separated from Mikania micrantha by HSCCC using the lower phase of this solvent system as mobile phase in one run. This study pave a simple and efficient method that could be used in the following separation of flavonoids from invasive plants.

Key words: Mikania micrantha (M. micrantha), flavonoids, high speed countercurrent chromatography (HSCCC), macroporous resin, separation

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