色谱 ›› 2017, Vol. 35 ›› Issue (6): 669-673.DOI: 10.3724/SP.J.1123.2017.01023

• 技术与应用 • 上一篇    

微波提取-高效液相色谱法测定大气颗粒物中的多环芳烃

王美飞, 杨丽莉, 胡恩宇   

  1. 南京市环境监测中心站, 江苏 南京 210013
  • 收稿日期:2017-01-19 出版日期:2017-06-08 发布日期:2013-09-29
  • 通讯作者: 王美飞,Tel:(025)83336949,E-mail:xjmf1290@sina.com.
  • 基金资助:

    南京市科学技术局人才培养项目(200905019);南京市环境保护局科研项目(200505).

Determination of polycyclic aromatic hydrocarbons in airborne particles by microwave extraction-high performance liquid chromatography

WANG Meifei, YANG Lili, HU Enyu   

  1. Nanjing Environmental Monitoring Center, Nanjing 210013, China
  • Received:2017-01-19 Online:2017-06-08 Published:2013-09-29
  • Supported by:

    Talent Cultivation Project of Nanjing Science and Technology Bureau (No. 200905019); Scientific Research Project of Nanjing Environmental Protection Bureau (No. 200505).

摘要:

建立了一种微波提取-高效液相色谱法测定大气颗粒物中多环芳烃的方法。吸附有颗粒物的超细玻璃纤维/石英纤维滤膜用正己烷/丙酮(1:1,v/v)混合溶剂经微波提取,提取液用弗罗里硅土柱净化,经浓缩定容后,采用液相色谱法-二极管阵列(PDA)-荧光双检测器测定。目标化合物在20.0~500 μg/L范围内线性良好,相关系数不小于0.9960。空白膜加标结果显示,目标化合物的回收率在56.3%~101%之间。该法已运用于南京市大气颗粒物中多环芳烃分布的调查研究。

关键词: 多环芳烃, 高效液相色谱法, 颗粒物, 微波提取

Abstract:

A method by using microwave extraction and high performance liquid chromatography was developed to determine polycyclic aromatic hydrocarbons (PAHs) in airborne particles. The glass/quartz fiber filter, on which particles adsorbed, was extracted with a mixed solvent n-hexane/acetone (1:1, v/v) using microwave, and the subsequent purification of the extract was carried out by a florisil cartridge. The prepared sample was determined by high performance liquid chromatography coupled with photodiode array (PDA) and fluorescence dual-detector. The target compounds showed good linearity between the mass concentration of 20.0 μg/L and 500 μg/L, with the correlation coefficients not less than 0.9960. The spiked recoveries of target compounds in blank filter ranged from 56.3%-101%. The current method has been applied to investigate the distribution of PAHs in airborne particles in Nanjing City.

Key words: high performance liquid chromatography (HPLC), microwave extraction, particles, polycyclic aromatic hydrocarbons (PAHs)

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