色谱

色谱

• 研究论文 • 上一篇    下一篇

反相高效液相色谱法同时测定保健品中的四种类胡萝卜素

李赫,陈敏,朱蕾,刘丽娟,王静钰   

  1. College of Food Science &Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • 收稿日期:2005-09-27 修回日期:2006-05-19 出版日期:2006-09-30 发布日期:1986-09-25
  • 通讯作者: 陈敏

Simultaneous Determination of Four Carotenoids in Health Products by Reversed-Phase High Performance Liquid Chromatography

LI He, CHEN Min, ZHU Lei, LIU Lijuan, WANG Jingyu
   

  1. College of Food Science &Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Received:2005-09-27 Revised:2006-05-19 Online:2006-09-30 Published:1986-09-25
  • Contact: CHEN Min

PDF

623

被引次数

32

摘要： 建立了不同剂型保健品中类胡萝卜素的提取方法和同时测定保健品中4种类胡萝卜素含量的高效液相色谱分析方法。采用DiamonsilTM C18色谱柱(250 mm×4.6 mm i.d.，5 μm),以乙腈和乙酸乙酯为流动相进行梯度洗脱,洗脱程序：先以100%乙腈洗脱15 min,然后乙酸乙酯的含量在10 min 内从0增加到100%,再以100%乙腈洗脱5 min;流速1 mL/min；采用二极管阵列检测器检测,检测波长为450 nm。以外标法定量,番茄红素、叶黄素、玉米黄素、β-胡萝卜素4种组分的线性关系良好,相关系数为0.9994～0.9997,检测限为0.4～0.5g/L。片剂样品、粉状样品、油状样品的加标回收率分别为95.3%～98.7%,93.7%～98.8%,97.1%～99.2%,相对标准偏差（RSD）分别为0.89%～2.0%,0.89%～2.8%,0.42%～1.2%。该法简便、快速、准确,是保健品中多种类胡萝卜素定量测定的可靠方法之一。

关键词: β-胡萝卜素, 保健品 , 番茄红素, 反相高效液相色谱, 类胡萝卜素, 叶黄素, 玉米黄素

Abstract: A method was established for the determination of carotenoids （lutein, zeaxanthin, lycopene, β-carotene） in health products by reversed-phase high performance liquid chromatography (RP-HPLC). The extraction methods of carotenoids from different types of samples were also established. Separation and quantification was achieved by using a DiamonsilTM C18 column (250 mm×4.6 mm i.d., 5 μm), and a mobile phase of acetonitrile and ethyl acetate were used with the following gradient elution: 100% acetonitrile at the first 15 min, then ethyl acetate increased to 100% in the next 10 min, at last 100% acetonitrile for 5 min with a flow rate of 1 mL/min, temperature of 25 ℃ and monitored with a photodiode array detector at 450 nm. The external standard calibration curves were used for quantification. There were good linear correlations for the four carotenoids. The correlation coefficients were between 0.9994 and 0.9997. The detection limits were 0.4-0.5 mg/L. The recoveries of four carotenoids in tablet samples were 95.3%-98.7% with the relative standard deviations (RSDs) of 0.89%-2.0%. The recoveries of four carotenoids in powder samples were 93.7%-98.8% with RSDs of 0.89%-2.8%. The recoveries of four carotenoids in oil samples were 97.1%-99.2% with RSDs of 0.42%-1.2%. This method is simple, fast, accurate and convenient. It can be used as one of the reliable means for quantitative determination of carotenoid in health products.

Key words: carotenoids, health products , lutein, lycopene, zeaxanthin, β-carotene, reversed-phase high performance liquid chromatography (RP-HPLC)