色谱

色谱

• 研究论文 • 上一篇    下一篇

高效液相色谱法同时测定黄芪中的五种异黄酮类成分

王晓辉 刘涛 李清 陈晓辉 毕开顺   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • 收稿日期:2005-10-27 修回日期:2005-12-16 出版日期:2006-09-30 发布日期:1986-09-25
  • 通讯作者: 毕开顺

Simultaneous Determination of Five Isoflavonoids in Commercial Radix Astragali by High Performance Liquid Chromatography

WANG Xiaohui, LIU Tao, LI Qing, CHEN Xiaohui, BI Kaishun   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2005-10-27 Revised:2005-12-16 Online:2006-09-30 Published:1986-09-25
  • Contact: BI Kaishun

PDF

1137

被引次数

136

摘要： 对蒙古黄芪中5种异黄酮类成分的含量进行了反相高效液相色谱法测定。色谱柱为Diamonsil C18柱,流动相为乙腈-水系统,梯度洗脱,检测波长230 nm,柱温35 ℃。毛蕊异黄酮-7-O-β-D-葡萄糖苷在20.12～201.2 mg/L、芒丙花素-7-O-β-D-葡萄糖苷在4.62～46.2 mg/L、9,10-二甲氧基紫檀烷-3-O-β-D-葡萄糖苷在4.86～48.6 mg/L、毛蕊异黄酮在9.24～92.4 mg/L、芒丙花素在6.92～69.2 mg/L时峰面积与浓度呈良好的线性关系,相关系数分别为0.9992,0.9997,0.9997,0.9995和0.9995。5种成分的加样回收率均高于94%,相对标准偏差（RSD）小于3.2%(n=9)。该法简便快速,重复性良好,结果准确可靠,可用于黄芪药材中5种主要异黄酮类成分的含量测定。

关键词: 反相高效液相色谱法, 黄芪 , 异黄酮

Abstract: A reversed-phase high performance liquid chromatographic method (RP-HPLC) was developed for the simultaneous analysis of five isoflavonoids of Huangqi, the roots of Astragalus mongholicus. HPLC determination was performed on a Diamonsil C18 column (200 mm × 4.6 mm i.d., 5 μm) and detected at 230 nm. The mobile phase consisted of (A)acetonitrile and (B)water. Gradient program was adopted as follows: 0-25 min from 17%A to 31%A, 25-45 min 35%A. The flow rate was maintained at 1.0 mL/min. The method was proved to be linear in the ranges of 20.12-201.2 mg/L (r=0.9992), 4.62-46.2 mg/L (r=0.9997), 4.86-48.6 mg/L (r=0.9997), 9.24-92.4 mg/L (r=0.9995) and 6.92-69.2 mg/L (r=0.9995)for the five isoflavonoids, calycosin-7-O-β-D-glucoside, formononetin-7-O-β-D-glucoside, 9,10-dimethoxypterocarpan-3-O-β-D-glucoside, calycosin and formononetin, respectively. The recoveries were greater than 94%, and relative standard deviations were less than 3.2%. The method has been successfully applied to the simultaneous determination of the five isoflavonoids of radix astragali.

Key words: isoflavonoid, radix astragali , reversed-phase high performance liquid chromatography (RP-HPLC)