色谱

色谱

• 研究论文 • 上一篇    下一篇

反相高效液相色谱法同时分离测定杜仲雄花及其产品中的
京尼平苷酸和绿原酸

董娟娥1,2,马希汉1

  

  1. 1.Northwest A & F University, the Key Laboratory of Exploitation and Utilization of Plant Resources in Shaanxi
    Province, Yangling 712100, China; 2.Institute of Soil and Water Conservation of the Ministry of
    Water Resources, Chinese Academy of Sciences, Yangling 712100, China
  • 收稿日期:2006-08-21 修回日期:2006-10-19 出版日期:2007-03-30 发布日期:1985-12-25
  • 通讯作者: 董娟娥

Determination of Geniposidic Acid and Chlorogenic Acid in Male
Flowers and Related Products of Eucommia ulmoides by
Reversed-Phase High Performance Liquid Chromatography

DONG Juane1,2, MA Xihan1   

  1. 1.Northwest A & F University, the Key Laboratory of Exploitation and Utilization of Plant Resources in Shaanxi
    Province, Yangling 712100, China; 2.Institute of Soil and Water Conservation of the Ministry of
    Water Resources, Chinese Academy of Sciences, Yangling 712100, China
  • Received:2006-08-21 Revised:2006-10-19 Online:2007-03-30 Published:1985-12-25

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摘要：

建立了利用反相高效液相色谱法（RP-HPLC）同时测定杜仲雄花及其产品中京尼平苷酸和绿原酸的方法。所用的色谱柱为Shim-pack VP-ODS(150 mm×4.6 mm，5 μm),流动相组成为甲醇-水-乙酸(体积比为24∶75∶1),检测波长为240 nm。在该色谱条件下,京尼平苷酸的含量在0.025~0.400 g/L、绿原酸的含量在0.075~1.200 g/L范围内线性关系良好,相关系数分别为0.9997和0.9999;加标回收率分别为100.2%和100.5%。该法适用于杜仲雄花及其产品中这2种成分的含量分析。

关键词: 杜仲, 反相高效液相色谱法, 京尼平苷酸, 绿原酸, 雄花, 雄花茶

Abstract:

A simple and rapid high performance liquid chromatographic method has been developed for the determination of geniposidic acid and chlorogenic acid in the male flowers and related products of Eucommia ulmoides. Two components were separated by a Shim-pack VP-ODS column (150 mm×4.6 mm, 5 μm) with a mobile phase of methanol-water-acetic acid (24∶75∶1, v/v) at a flow rate of 1 mL/min, column temperature of 30 ℃ and detection wavelength of 240 nm. Under the chromatographic conditions mentioned above, the method performance, such as the number of theoretical plate, resolution, trailing etc have all reached required level. The linear ranges were 0.025-0.400 g/L for geniposidic acid and 0.075-1.200 g/L for chlorogenic acid, with the correlation coefficients of 0.9997 and 0.9999, respectively. The average recoveries were 100.2% and 100.5%, and the relative standard deviations (RSDs) were 1.47% and 1.49% respectively. The minimum detection limits were 0.02 μg/L for geniposidic acid, and 0.06 μg/L for chlorogenic acid. The method developed has demonstrated the characteristics of simple mobile phase composition, short retention, good resolution, high repeatability and precision. It is suitable for the determination of the two compounds in the male flowers of E. ulmoides and related products.

Key words: chlorogenic acid, Eucommia ulmoides, geniposidic acid, male flower, male flower tea , reversed-phase high performance liquid chromatography(RP-HPLC)