色谱

色谱

• 研究论文 • 上一篇    下一篇

树兰花挥发性成分的提取及鉴定

张峻松1,姚二民1,王建民1,徐如彦1,2

  

  1. 1.College of Food and Bioengineering, Zhengzhou Institute of Light Industry, Zhengzhou 450002, China;
    2.Xuzhou Cigarette Factory, Xuzhou 221005, China
  • 收稿日期:2006-10-15 修回日期:2006-11-20 出版日期:2007-05-30 发布日期:1985-09-25
  • 通讯作者: 张峻松

Extraction and Identification of Volatile Constituents in the Flowers of Aglaia odorata Lour.

ZHANG Junsong1, YAO Ermin1, WANG Jianmin1, XU Ruyan1,2
  

  1. 1.College of Food and Bioengineering, Zhengzhou Institute of Light Industry, Zhengzhou 450002, China;
    2.Xuzhou Cigarette Factory, Xuzhou 221005, China
  • Received:2006-10-15 Revised:2006-11-20 Online:2007-05-30 Published:1985-09-25

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摘要：

采用超临界CO2流体从树兰花中提取挥发油,得油率为2.64%。利用气相色谱-质谱联用仪(GC-MS)对树兰花油中的化学成分进行分离和鉴定,共分离出54种组分,确认了其中的48种成分,其中有18种萜烯类化合物和12种酯类物质等成分,如α-蛇麻烯、亚麻酸乙酯、大根香叶烯-D、β-榄香烯、古巴烯、石竹烯、茉莉酮酸甲酯、β-蛇麻烯-7-醇和棕榈酸乙酯等。

关键词: 超临界CO2流体萃取法 , 挥发油, 气相色谱-质谱, 树兰花

Abstract:

The volatile constituents in the flowers of Aglaia odorata were extracted by supercritical CO2 fluid under 25 MPa and 40 ℃ for 80 min. The oil yield was 2.64%. These volatile constituents were separated and identified by capillary gas chromatography-mass spectrometry (GC-MS). Experiments were performed on an Agilent 6890 GC Chromatograph with Agilent 5973N Mass Selective Detector. The GC separation conditions was carried out on an HP-5MS capillary column (60 m×250 μm, 0.25 μm); oven temperature, 50 ℃ (held for 2 min) to 280 ℃ at a rate of 5 ℃/min, and held for 8 min; split ratio, 1∶10; injector temperature, 270 ℃. Mass spectra were collected in the scan range of m/z 50-550. The measurements were performed with electron bombardment ion （EI） source with electron energy of 70 eV and electron multiplier voltage of 1.65 kV. The results showed that 54 peaks were separated and 48 compounds were identified for the essential oil extracted from Aglaia odorata L. There were 18 terpenes, 12 esters and other constituents in the volatile oil fraction. The constituents in the oil fraction were α-humulene, followed by ethyl linolenate, germacrene D, β-elemene, copaene, caryophyllene, methyl jasmonate, β-humulene-7-ol, ethyl palmitate, etc.

Key words: Aglaia odorata L., supercritical CO2 fluid extraction , volatile oil, gas chromatography-mass spectrometry (GC-MS)