色谱

色谱

• 研究论文 • 上一篇    下一篇

固相萃取-高效液相色谱法同时测定啤酒中的4种异构化α-酸

李崎1,周天1,顾国贤2

  

  1. 1.Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University,
    Wuxi 214036, China; 2.School of Biotechnology, Southern Yangtze University, Wuxi 214036, China
  • 收稿日期:2007-01-09 修回日期:2007-03-26 出版日期:2007-07-30 发布日期:1985-06-25
  • 通讯作者: 李崎

Determination of Isomerized α-Acids in Beer Using Solid Phase
Extraction-High Performance Liquid Chromatography

LI Qi1, ZHOU Tian1, GU Guoxian2   

  1. 1.Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University,
    Wuxi 214036, China; 2.School of Biotechnology, Southern Yangtze University, Wuxi 214036, China
  • Received:2007-01-09 Revised:2007-03-26 Online:2007-07-30 Published:1985-06-25

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摘要：

建立了固相萃取-高效液相色谱同时测定啤酒中4种异构化α-酸的方法。采用Sep-Pak C18萃取柱,系统研究了啤酒中异构化α-酸的最佳固相萃取条件。选择以2 mL酸化甲醇为洗脱溶剂,萃取前调啤酒样品的pH至2.5。该方法准确可靠,重现性好,4种异构化α-酸的回收率为90.6%～96.4%,相对标准偏差小于4%。异α-酸、二氢异α-酸、四氢异α-酸和六氢异α-酸的最低检测限依次为0.14,0.36,0.33和0.53 mg/L。

关键词: 高效液相色谱法, 固相萃取, 啤酒 , 异构化α-酸

Abstract:

An analytical method for four isomerized α-acids in beer was developed using solid phase extraction (SPE) coupled with high performance liquid chromatography (HPLC). The method for solid phase extraction was systematically optimized. The optimal conditions were determined as follows: 2 mL of acidic methanol as eluting solvent through a Sep-Pak C18 cartridge and the pH value of beer was adjusted to 2.5. Under these conditions, the recoveries of four isomerized α-acids for spiked standards (n=10) were 90.6%-96.4% and the relative standard deviations (RSDs) were generally less than 4%. The detection limits for iso-α-acid, rho-iso-α-acid, tetrahydro-iso-α-acid and hexahydro-iso-α-acid were 0.14 mg/L, 0.36 mg/L, 0.33 mg/L and 0.53 mg/L, respectively. The method is sensitive, accurate and suitable for the analysis of isomerized α-acids in beer.

Key words: beer , high performance liquid chromatography (HPLC), isomerized α-acid, solid phase extraction (SPE)