色谱

色谱

• 研究论文 • 上一篇    下一篇

高效液相色谱-串联质谱法分离鉴定绿原酸及其相关杂质

田晨煦1,徐小平1,廖丽云2,张洁1,刘静1,周莎1   

  1. 1.West China Pharmaceutical School of Sichuan University, Chengdu 610041, China;
    2.Chengdu Medical College, Chengdu 610083, China
  • 收稿日期:2007-01-15 修回日期:2007-04-29 出版日期:2007-07-30 发布日期:1985-06-25
  • 通讯作者: 徐小平

Separation and Identification of Chlorogenic Acid and Related Impurities by High Performance Liquid Chromatography-Tandem Mass Spectrometry

TIAN Chenxu1, XU Xiaoping1, LIAO Liyun2, ZHANG Jie1, LIU Jing1, ZHOU Sha1   

  1. 1.West China Pharmaceutical School of Sichuan University, Chengdu 610041, China;
    2.Chengdu Medical College, Chengdu 610083, China
  • Received:2007-01-15 Revised:2007-04-29 Online:2007-07-30 Published:1985-06-25

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摘要： 建立了高效液相色谱-串联质谱法（HPLC-MS/MS)分离和鉴定绿原酸及其相关杂质的方法。采用C18色谱柱(5 μm,4.6 mm×150 mm),乙腈-水（含0.1%甲酸）(体积比为8∶92)为流动相,经HPLC-MS/MS和HPLC-二极管阵列检测器在线检测,对工业绿原酸中的奎尼酸、咖啡酸、绿原酸同分异构体等8个相关杂质的结构进行了鉴定。

关键词: 高效液相色谱-串联质谱法, 鉴别 , 绿原酸, 相关杂质

Abstract:

A method using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established for analysis and identification of chlorogenic acid along with its related impurities. A Gemini C18 HPLC column (4.6 mm×150 mm，5 μm) was used with acetonitrile-water ( containing 0.1% formic acid) (8∶92, v/v) as mobile phase. Eight related impurities of chlorogenic acid were identified and their structures were determined by using on-line HPLC-MS/MS and photodiode array detector (DAD). The method is quick, simple and can be used directly to identify the structure of unknown trace substances in the sample of chlorogenic acid.

Key words: chlorogenic acid, identification
, related impurities, high performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS)