色谱

色谱

• 研究论文 • 上一篇    下一篇

微波萃取-气相色谱法测定血液中的甲基苯丙胺

孙洪锋1,谷学新1,王继芬2,倪为贵1,李文君2,李瑛2

  

  1. 1.Department of Chemistry, Capital Normal University, Beijing 100037, China;
    2.Chinese People’s Public Security University, Beijing 100038, China
  • 收稿日期:2007-01-30 修回日期:2007-04-11 出版日期:2007-07-30 发布日期:1985-06-25
  • 通讯作者: 谷学新

Determination of Methamphetamine in Human Blood
Using Microwave Extraction-Gas Chromatography

SUN Hongfeng1, GU Xuexin1, WANG Jifen2, NI Weigui1, LI Wenjun2, LI Ying2   

  1. 1.Department of Chemistry, Capital Normal University, Beijing 100037, China;
    2.Chinese People’s Public Security University, Beijing 100038, China
  • Received:2007-01-30 Revised:2007-04-11 Online:2007-07-30 Published:1985-06-25

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摘要：

建立了人体血液中甲基苯丙胺的微波萃取-气相色谱测定方法。分别考察了萃取溶剂种类、用量、样品pH值以及萃取温度、时间等因素对萃取率的影响，并与液-液萃取法进行比较。结果表明,在相同条件下,微波萃取率高于液-液萃取。血液中甲基苯丙胺的最佳提取条件为:调节血样pH为13,以乙酸乙酯为萃取溶剂,于30 ℃下微波提取 8 min。在此条件下平均萃取率达到81.4%,相对标准偏差为6.4%(n=5)。提取液经气相色谱-氢火焰离子化检测器检测,甲基苯丙胺和基体之间得到了很好的分离，对血液中甲基苯丙胺的最低检测限为220 μg/L。该方法是一种快速、准确、灵敏的测定血液中甲基苯丙胺的方法。

关键词: 甲基苯丙胺, 气相色谱, 微波萃取, 血液

Abstract:

A method was developed for the determination of methamphetamine (MAM) in human blood using microwave extraction-gas chromatography (GC). To improve the extraction efficiency, experimental parameters on the extraction, including such as extraction solvent and its amount, pH value of blood sample, extraction time and temperature were investigated. Comparing with conventional liquid-liquid extraction method, the microwave extraction showed better efficiency under the optimal conditions. The optimal conditions were as follows: the pH of blood sample at 13, ethyl acetate as extraction solvent, extraction at 30 ℃ for 8 min. The average recovery of MAM with this extraction method was 81.4%, and the relative standard deviation was 6.4%. The limit of detection was 220 μg/L for MAM in the blood. Using this method, MAM need not be derivatized and can be separated from the matrix. The results indicate that the developed method is rapid, accurate and sensitive, and can be used for the determination of MAM in blood samples.

Key words: blood , gas chromatography (GC), methamphetamine, microwave extraction