色谱

色谱

• 研究论文 • 上一篇    下一篇

高效体积排阻色谱法测定改性柑橘果胶的分子质量

张文博1,2,高林1,施秀芳1,张启来3

  

  1. 1.School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China;
    2.Tianjin Union Biotechnology Development Co., Ltd., Tianjin 300457, China; 3.Environmental Protection Bureau of Wuqing District, Tianjin 301700, China
  • 收稿日期:2007-04-05 修回日期:2007-06-29 出版日期:2007-09-30 发布日期:1985-03-25
  • 通讯作者: 张文博

Determination of the Molecular Mass of Modified Citrus Pectin Using High Performance Size Exclusion Chromatography

ZHANG Wenbo1,2, GAO Lin1, SHI Xiufang1, ZHANG Qilai3   

  1. 1.School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China;
    2.Tianjin Union Biotechnology Development Co., Ltd., Tianjin 300457, China; 3.Environmental Protection Bureau of Wuqing District, Tianjin 301700, China
  • Received:2007-04-05 Revised:2007-06-29 Online:2007-09-30 Published:1985-03-25

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摘要：

改性柑橘果胶(MCP)是一种具有抗肿瘤细胞生长、侵袭和转移活性的多糖。由于多糖的分子质量对其活性有很大的影响,因而需要测定其分子质量。选择的色谱条件为：TSK G3000PWXL色谱柱,以50 mmol/L醋酸铵缓冲液为流动相,流速为0.5 mL/min,柱温为40 ℃,样品质量浓度为10 g/L,进样体积为20 μL,示差折光检测器检测。采用Pullulan糖标准品,经校正获得回归方程,通过回归方程计算得到的MCP平均相对分子质量为:Mn为21000，Mp为46000,Mw为43000,Mz为66000。MCP的多分散性指数(PD)为2。该方法具有良好的重现性,而且简便、快速,为MCP的生产工艺、质量控制和组效关系等的研究提供了实用的分析检测手段。

关键词: 多糖, 分子质量 , 改性柑橘果胶, 高效体积排阻色谱

Abstract:

Modified citrus pectin (MCP) is a polysaccharide with anti-tumor growth, anti-invasiveness and anti-metastasis activities. It is of significance to determine the molecular mass and polydispersity of polysaccharide, which could influence pharmacological activities of polysaccharide. To the best of our knowledge, no study has been reported on calculating the molecular mass of MCP by high performance size exclusion chromatography-refractive index detection (HPSEC-RID). A TSK G3000PWXL column was used at 40 ℃, and the mobile phase was composed of 50 mmol/L NH4Ac solution. Pullulan with known molecular mass was chosen as calibration standard to obtain regression equation. Relative molecular mass of MCP can be derived from the regression equation. Relative molecular masses of MCP were obtained: Mn, 21000; Mw, 43000; Mp, 46000; Mz, 66000. The polydispersity index of MCP was 2. The established method is simple, rapid and repeatable for quality control in preparing MCP and it would be also applicable to the study of composition-activity relationship of MCP in the future.

Key words: modified citrus pectin （MCP）, molecular mass , polysaccharide, high performance size exclusion chromatography （HPSEC）