关键词: DL-氨基酸, L-脯氨酸, 单分散树脂, 对映体分离 , 手性配体交换色谱固定相

Abstract:

L-Proline chiral stationary phase for ligand exchange chromatography was prepared in the following steps. First, the particles were completely hydrolyzed. Second, the hydrolyzed particles reacted with epichlorhydrin to obtain chlorinated beads. Third, the chlorinated beads reacted with L-proline. The chiral stationary phase was characterized by elemental analysis. Chromatographic resolutions of DL-amino acids were achieved on the chiral stationary phases by using 0.1 mol/L NaAc with 0.1 mmol/L Cu(Ac)2 solution as mobile phase and detection at 254 nm. The elution order of D-isomer before L-isomer was observed for all the DL-amino acids resolved except DL-proline. For DL-proline the elution order was different from the others because of its five membered ring structure. The influences of the mobile phase pH, concentration of Cu(Ⅱ), flow rate of eluent and temperature of column on the resolution of DL-amino acids by ligand exchange chromatography were investigated for the optimization of chromatographic conditions. The results showed that enantioseparation of some DL-amino acids was performed by ligand exchange chromatography on this chiral stationary phase with satisfactory results.

Key words: DL-amino acids, enantiomeric separation , L-proline, monodisperse resin, chiral ligand exchange chromatographic stationary phase