色谱

色谱

• 研究论文 • 上一篇    下一篇

液相色谱-质谱法同时鉴别中药制剂中的15种糖皮质激素

夏瑞,车宝泉,张喆

  

  1. Beijing Municipal Institute of Drug Control, Beijing 100035, China
  • 收稿日期:2007-05-08 修回日期:2007-08-27 出版日期:2007-11-30 发布日期:1984-12-25
  • 通讯作者: 夏瑞

Simultaneous Identification of Fifteen Glucocorticoids in Chinese Traditional Medicine Preparations by Liquid Chromatography-Mass Spectrometry

XIA Rui, CHE Baoquan, ZHANG Zhe   

  1. Beijing Municipal Institute of Drug Control, Beijing 100035, China
  • Received:2007-05-08 Revised:2007-08-27 Online:2007-11-30 Published:1984-12-25
  • Contact: XIA Rui

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摘要：

为了打击药物的非法滥用,建立起鉴别中药制剂中可能添加的糖皮质激素的液相色谱-质谱分析方法。针对不同的中药剂型,采用不同的试样处理方式。提取后的样品溶液采用Diamond C18柱(5 μm,250 mm×4.6 mm)分离,以流动相A（水-四氢呋喃，体积比为100∶1）和B（乙腈-水-四氢呋喃，体积比为80∶20∶1）进行梯度洗脱。通过与对照品保留时间、一级质谱及二级质谱图的对比进行定性。应用该法鉴定了中药制剂中的糖皮质激素,在供试品溶液中检出了醋酸泼尼松。

关键词: 糖皮质激素, 液相色谱-质谱法, 中药制剂

Abstract:

To monitoring illegal abuse of these drugs, a liquid chromatography-mass spectrometry (LC-MS) method for identification of glucocorticoids in Chinese traditional medicine preparations was developed. Samples were extracted using different methods according to the nature of products. The analyses were performed on a Diamond C18 column (5 μm, 250 mm×4.6 mm) with gradient elution using water-tetrahydrofuran (100∶1, v/v) as mobile phase A and acetonitrile-water-tetrahydrofuran (80∶20∶1, v/v) as mobile phase B. Prednisone acetate in the preparation used to cure dermatitis was identified by comparing the retention time, full scan MS, and full scan MS2.

Key words: Chinese traditional medicine preparations , glucocorticoids, liquid chromatography-mass spectrometry (LC-MS)