色谱

色谱

• 研究论文 • 上一篇    下一篇

多孔石墨化碳柱分析强极性化合物三七素

付春梅,刘三康,李章万

  

  1. West China School of Pharmacy, Sichuan University, Chengdu 610041, China
  • 收稿日期:2007-06-08 修回日期:2007-09-17 出版日期:2007-11-30 发布日期:1984-12-25
  • 通讯作者: 李章万

Determination of Dencichine in Sanchi Using Liquid Chromatography on Porous Graphitic Carbon Column

FU Chunmei, LIU Sankang, LI Zhangwan   

  1. West China School of Pharmacy, Sichuan University, Chengdu 610041, China
  • Received:2007-06-08 Revised:2007-09-17 Online:2007-11-30 Published:1984-12-25

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摘要：

建立了强极性的非蛋白氨基酸三七素在石墨化碳色谱柱上的反相高效液相色谱分析方法。以Thermo Hypercarb石墨化碳柱(100 mm×4.6 mm,5 μm)为分离柱,以0.05 mol/L NaH2PO4(H3PO4调pH 2.2)为流动相,流速为1 mL/min,于柱温80 ℃、检测波长220 nm条件下分离检测。对三七素在石墨化碳柱上的保留及其影响因素的研究结果表明:三七素在石墨化碳柱上的保留机理仍主要是疏水相互作用。将建立的色谱条件用于三七药材中三七素的测定,进样量在0.22～4.4 μg范围内线性关系良好(r=0.9999),平均加标回收率为99.5%(n=9),相对标准偏差不高于2.2%,分析速度快(三七素的保留时间不到3 min)。

关键词: 保留机理, 多孔石墨化碳柱, 三七 , 三七素

Abstract:

A simple reversed-phase high performance liquid chromatographic method was developed for the determination of dencichine in sanchi on a porous graphitic carbon (PGC) column. The effects of different buffers, buffer concentrations, pH values of mobile phase and mobile phase compositions as well as column temperatures on the retention of dencichine were studied. The retention mechanism of dencichine on a porous graphitic carbon column seems to be mainly hydrophobic interaction according to the study. The chromatographic method developed was used to determine dencichine in sanchi. The calibration curve was linear in the range of about 0.22-4.4 μg (r=0.9999) and the recovery for the extract was 99.5% with the relative standard deviation of 2.2% (n=9).

Key words: dencichine, retention mechanism, sanchi , porous graphitic carbon column