色谱

色谱

• 研究论文 • 上一篇    下一篇

高效液相色谱-电喷雾串联质谱法同时测定鸡肉中残留的磺胺类和氟喹诺酮类兽药

刘芃岩1,姜宁1,3,王英峰2,晏利芝2

  

  1. 1.College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China;
    2.The Department of Chemistry, Capital Normal University, Beijing 100037, China;
    3.Langfang Entry-Exit Inspection and Quarantine Bureau, Langfang 065000, China
  • 收稿日期:2007-08-30 修回日期:2007-10-26 出版日期:2008-05-30 发布日期:1984-03-25
  • 通讯作者: 刘芃岩

Simultaneous determination of sulfonamides and fluoroquinolones residues in chicken by high performance liquid
chromatography-electrospray tandem mass spectrometry

LIU Pengyan1, JIANG Ning1,3, WANG Yingfeng2, YAN Lizhi2

  

  1. 1.College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China;
    2.The Department of Chemistry, Capital Normal University, Beijing 100037, China;
    3.Langfang Entry-Exit Inspection and Quarantine Bureau, Langfang 065000, China
  • Received:2007-08-30 Revised:2007-10-26 Online:2008-05-30 Published:1984-03-25

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摘要：

建立了一种同时测定鸡肉中两类共10种兽药(3种磺胺和7种氟喹诺酮类药物)残留量的高效液相色谱-电喷雾串联质谱方法(HPLC
-ESI-MS2)。样品经2%醋酸-乙腈提取,正己烷脱脂,过ENVI-18固相萃取柱净化,经氮气吹干后,残余物用流动相定容到1 mL。以乙腈和
0.05%甲酸溶液作为流动相,采用梯度洗脱程序进行液相色谱分离,用质谱检测器进行定性和定量分析,并对10种药物的二级质谱碎裂方
式进行分析。10种药物在0.02～2.0 mg/L范围内线性良好,相关系数均大于0.9988。检出限(LOD)为1.10～6.85 μg/kg,定量限(LOQ)
为3.68～22.85 μg/kg,样品的平均加标回收率为68.9%～102.6%,相对标准偏差均小于8.6%(n=3)。实验结果表明,该方法灵敏度高,重
现性好,确证能力强,分析时间短,可满足动物源性食品中磺胺和氟喹诺酮类药物的残留分析。

关键词: 电喷雾串联质谱, 动物源性食品 , 氟喹诺酮, 高效液相色谱, 磺胺

Abstract:

1.College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China;
2.The Department of Chemistry, Capital Normal University, Beijing 100037, China;
3.Langfang Entry-Exit Inspection and Quarantine Bureau, Langfang 065000, China

Key words:
sulfonamides, animal food , electrospray tandem mass spectrometry (ESI-MS2), fluoroquinolones, high performance liquid chromatography (HPLC)