色谱

色谱

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从猪血中分离纯化高纯度的猪血红蛋白

周勃,边六交

  

  1. College Life Science, Northwest University, National Engineering Research Center for Miniaturized Detection System,
    Xi’an 710069, China
  • 收稿日期:2007-08-14 修回日期:2007-10-22 出版日期:2008-05-30 发布日期:1984-03-25
  • 通讯作者: 边六交

Separation and purification of porcine hemoglobin from porcine blood

ZHOU Bo, BIAN Liujiao   

  1. College Life Science, Northwest University, National Engineering Research Center for Miniaturized Detection System,
    Xi’an 710069, China
  • Received:2007-08-14 Revised:2007-10-22 Online:2008-05-30 Published:1984-03-25

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摘要：

为了从猪血中分离纯化高纯度的猪血红蛋白,建立了通过超滤、DEAE-Sepharose Fast Flow离子交换色谱和Sephadex G-75凝胶
排阻色谱三步法制备高纯度猪血红蛋白的方法,并通过十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、高效凝胶排阻色谱和反相高
效液相色谱方法,对纯化后的猪血红蛋白进行了鉴定。经三步分离纯化后,猪血红蛋白的纯度大于99%,含量为1.328 g/L。

关键词: 超滤, 纯化 , 分离, 凝胶排阻色谱, 阴离子交换色谱, 猪血红蛋白

Abstract:

In order to perform the isolation and purification of porcine hemoglobin from porcine blood. A three-step
method, utilizing ultrafiltration, DEAE-Sepharose Fast Flow anion-exchange chromatography and Sephadex G-75 gel
exclusion chromatography in sequence, for the separation and purification of porcine hemoglobin was developed. The
obtained hemoglobin was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), high-
performance size-exclusion chromatography and reversed-phase high performance liquid chromatography. Through the
three-step purification process, the purity of the obtained hemoglobin was more than 99% with a concentration of
1.328 g/L.

Key words:
separation, anion-exchange chromatography, gel exclusion chromatography, porcine hemoglobin, purification , ultrafiltration