色谱

色谱

• 研究论文 • 上一篇    下一篇

正相模式/反相模式的二维液相色谱系统的构建与应用

兰韬;焦丰龙;唐涛;王风云;李彤;张维冰

  

  1. 1.Chemical Engineering Institute, Nanjing University of Science and Technology, Nanjing 210094, China;
    2.Dalian Elite Analytical Instruments Co., Ltd., Dalian 116023, China; 3.Animal Science and
    Veterinary Institute, Jilin University, Changchun 130012, China; 4.Dalian Institute of
    Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China
  • 收稿日期:2008-01-17 修回日期:1900-01-01 出版日期:2008-05-30 发布日期:1984-03-25
  • 通讯作者: 张维冰

Establishment and Application of Two-dimensional Liquid Chromatography System(NPLC／RPLC)

LAN Tao1,2, JIAO Fenglong3, TANG Tao1,2, WANG Fengyun1, LI Tong2, ZHANG Weibing4   

  1. 1.Chemical Engineering Institute, Nanjing University of Science and Technology, Nanjing 210094, China;
    2.Dalian Elite Analytical Instruments Co., Ltd., Dalian 116023, China; 3.Animal Science and
    Veterinary Institute, Jilin University, Changchun 130012, China; 4.Dalian Institute of
    Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China
  • Received:2008-01-17 Revised:1900-01-01 Online:2008-05-30 Published:1984-03-25
  • Contact: ZHANG Weibing

PDF

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摘要： 以4.6mm×50 mm i. d.的Hypersil SiO2正相色谱柱为第一维，4.6mm×250 mm i. d.的Kromasil C18反相色谱柱为第二维，通过升高第二维色谱温度的方法增加两维流动相间互溶性的方法构建了定量环-阀切换接口的二维液相色谱系统(NPLC×RPLC)。根据有机溶剂的特征，在第一维正相色谱流动相中加入二氧六环；第二维反相色谱流动相中加入异丙醇，在改善流动相兼容性的同时，有效调整分离选择性。采用此系统对正天丸样品进行分离分析，达到1120的峰容量。

关键词: 定量环-阀切换接口, 二维液相色谱系统, 正天丸

Abstract: A two-dimensional liquid chromatography (NPLC×RPLC) was developed. Normal phase liquid chromatography (NPLC) with a Hypersil SiO2 column (4.6mm×50 mm i. d.) was used as the first dimension, and reversed phase liquid chromatography (RPLC) with a Kromasil C18 column (4.6mm×250 mm i. d.) was used as the second dimension. The interface was based on a ten-port, two-position valve with a storage loop. As to improve the incompatibility of the two dimensional mobile phase and be good for adjusting the selectivity of separation, 1,4-Dioxane was used in the mobile phase of the first dimension, and isopropyl alcohol was used in the mobile phase of the second dimension based on the character of organic solvent. By rising the temperature of the second dimension, the system avoid the incompatibility of the two dimensional mobile phase. Zhengtian Wan was analyzed by this system, the total peak capacity reached 1120.

Key words: Sample loops-valve interface, Two-dimensional LC, Zhengtian Wan