色谱

色谱

• 技术与应用 • 上一篇    下一篇

高效液相色谱法同时测定化妆品中的呋喃妥因和呋喃唑酮

张庆*,王超,王星,武婷,马强   

  1. Institute of Industrial Product Inspection, Chinese Academy of Inspection and Quarantine, Beijing 100123, China
  • 收稿日期:2008-07-09 修回日期:2008-09-10 出版日期:2009-03-30 发布日期:1982-12-25
  • 通讯作者: 张庆
  • 基金资助:
    国家标准委员会计划项目(No. 20072831-T-607)

Simultaneous determination of nitrofurantoin and furazolidone in cosmetics using high performance liquid chromatography

ZHANG Qing*, WANG Chao, WANG Xing, WU Ting, MA Qiang   

  1. Institute of Industrial Product Inspection, Chinese Academy of Inspection and Quarantine, Beijing 100123, China
  • Received:2008-07-09 Revised:2008-09-10 Online:2009-03-30 Published:1982-12-25
  • Contact: ZHANG Qing

PDF

675

被引次数

24

摘要： 建立了一种同时检测化妆品中呋喃妥因和呋喃唑酮的高效液相色谱法(HPLC)。乳液、膏霜、化妆水、散粉、唇膏类等不同类型的化妆品样品由乙腈-甲醇(体积比为1∶1)混合溶液超声提取后进行HPLC分析。采用Kromasil C18色谱柱,以乙腈-0.4%乙酸溶液(体积比为30∶70)为流动相,流速1.0 mL/min,采用二极管阵列检测器检测,检测波长为365 nm;采用外标法定量,呋喃妥因和呋喃唑酮检测的线性范围为0.1～20 mg/L,相关系数为0.9999,最低检出限为1.2 mg/kg;在0.2,1.0,10.0 mg/L加标水平下,平均回收率为88.0%～104.6%,相对标准偏差为0.5%～4.8%。该方法快速准确,可用于化妆品中呋喃妥因和呋喃唑酮的同时测定。

关键词: 呋喃妥因, 呋喃唑酮, 高效液相色谱法, 化妆品

Abstract: A method was developed for the simultaneous determination of nitrofurantoin and furazolidone in cosmetics using reversed-phase high performance liquid chromatography. Cosmetics samples of various types, including lotion, cream, emollient, waterpowder and lipstick, were extracted under ultrasonication with acetonitrile-methanol (1∶1, v/v). A Kromasil C18 analytical column (250 mm×4.6 mm, 5 μm) was used for the separation at 25 ℃. Acetonitrile-0.4% acetic acid (30∶70, v/v) was used as the mobile phase at a flow rate of 1.0 mL/min. The detection was performed at 365 nm by a diode array detector. The quantitative analysis was performed using external standard calibration curve. The linear ranges for nitrofurantoin and furazolidone were 0.1-20 mg/L (r=0.9999). The average recoveries were 88.0%-104.6% with the relative standard deviations of 0.5%-4.8%. The method is convenient, rapid, reliable and suitable for the determination of nitrofurantoin and furazolidone in cosmetics.

Key words: cosmetics , furazolidone, nitrofurantoin, high performance liquid chromatography (HPLC)